Integrative Liver Cell 2017-07-28T09:40:54+00:00

Integrative Liver Cell Core

THE INTEGRATIVE LIVER CELL CORE (ILCC), formerly known as the Non-Parenchymal Liver Cell Core…

  • Strives to serve the scientific community of alcoholic liver disease (ALD) and cirrhosis via specialized services involving isolation of different liver cell types from normal rodents and rodent models of ALD and liver fibrosis.
  • Provides primary or stored hepatocytes (HC), hepatic macrophages/Kupffer cells (HM), hepatic stellate cells (HSC), liver sinusoidal endothelial cells (LSEC), liver mesothelial cells (MC), CD133+ liver tumor-initiating stem cell-like cells (TICs), and CD133+ liver progenitor cells, each isolated from mice or rats.
  • Supports specialized analysis such as cell lineage and fate tracing using Rosa-reporter mice; FACS-based isolation of quiescent vs. activated HSCs from Col1a1-GFP mice; infiltrating vs. resident HM and tracking on blood monocytes.
  • Offers Cell Bank, a collection of small aliquots of isolated cells or RNA from normal and diseased rodent livers for nominal fees – ideal for pilot analysis.

ILCC Cell diagram

A. Primary mouse HSCs and their vitamin A autofluorescence.
B. Primary rat HMs.
C. Primary mouse LSECs and their uptake of Acetylated-LDL.
D. Primary mouse MCs.

Since its inception in 2001, the ILCC has served 59 investigators from 18 institutions by performing 6,218 isolation preparations with an average of 444 preps per year, supporting 59 publications and acquisition or proposal of 50 NIH/VA/Foundation grants plus 7 pending applications. Collectively, these data attest that the ILCC contributes as a national resource by serving investigators across the nation.

The most powerful outcome ensues when these services are applied to the rodent models of ALD or liver fibrosis, allowing direct analysis of specific cellular changes in the evolution of the diseases. This approach is achieved by the ILCC’s close collaboration with the Animal Core of the NIAAA-funded Southern California Research Center for ALPD and Cirrhosis and allows isolation of the different cell types from diverse pathologic spectra. These models include the intragastric ethanol infusion (iG) models now reproducing the clinically relevant pathologic spectra of ALD, such as mild or severe chronic alcoholic steatohepatitis (ASH), with liver fibrosis and alcoholic neutrophilic hepatitis (AH).

ILCC brochure (Website version). Contact Gencie Turner, PhD at to receive hard copies.

REQUESTS FOR ROUTINE OR COMMON SERVICES

  1. Due to the large volume of service requests, and our primary mechanism of funding, priority will be given to researchers with projects relevant to alcohol.
  2. Requests for normal rat and mouse cells should be directed to Stephanie Pan (stephaqp@usc.edu or 323-442-3850) or Dr. Kinji Asahina (asahina@med.usc.edu) at least 1-3 weeks prior to the time the cells are needed
  3. Requests for cell isolation services from the disease models (as described under Animal Core Services), should be made to Stephanie Pan (stephaqp@usc.edu or 323-442-3850) or Dr. Asahina (asahina@med.usc.edu) in advance.
  4. The ILCC charges are based on a whole isolation procedure per rat or mouse and not on cell numbers isolated or attached for culture, because the cell yield and attachment vary.
  5. Click here for tables summarizing the yield of cells, purity, and viability for commonly isolated cell types, the data which are based on 20 most recent preps performed (updated May 2015).
  6. The services are rendered to Center or non-Center members on a charge back basis to recover the costs of supplies which are not supported by the Center and resource grants from NIAAA. See the chargeback fees in a table below for commonly performed services.
  7. For preliminary trials and young investigators or trainees, subsidized fees may be considered.  Consult Dr. Asahina (asahina@med.usc.edu). 50% discount for “F” and “K” awardees..
    Cell Isolation Services Rat Mouse
    Hepatic Macrophages (HM) $200 $150
    Hepatic Stellate Cells (HSC) $200 $150
    HM & HSC (from the same rodent) $300 $230
    Liver Sinusoidal Endothelial Cells (LSEC) $200 $150
    Hepatocytes (HC) $150 $100
    LSEC & HC (from the same rodent) $300 $200
    HM & Hepatocytes (from the same rodent) N/A $200
  8. Occasionally, the services can be rendered to those who are not included in the priority criteria, including industries, provided that the ILCC has the availability in its schedule.  Such services will have higher charge back rates, which include personnel costs and surcharges.
  9. If you wish to know the techniques used to isolate cells, please click here.

THE ILCC MANAGEMENT AND OPERATIONS

L to R: Raymond Wu, Kinji Asahina, Asma Deras, Stephanie Pan, Hidekazu Tsukamoto

Director: Hidekazu Tsukamoto, D.V.M., Ph.D. (htsukamo@med.usc.edu)
Associate Director: Kinji Asahina, Ph.D. (asahina@med.usc.edu)
Research Laboratory Specialist: Stephanie Pan (stephaqp@usc.edu)
Core Scientist: Raymond Wu, Ph.D. (raymonpw@usc.edu)
Core Financial Analyst (chargebacks): Asma Deras (asmadera@usc.edu)

REFERENCES CITED

  1. Lazaro R, Wu R, Lee S, Zhu NL, Chen CL, French SW, et al. Osteopontin deficiency does not prevent but promotes alcoholic neutrophilic hepatitis in mice. Hepatology. 2014. doi: 10.1002/hep.27383
    [doi].  PubMed PMID: 25132354; PubMed Central PMCID: PMC4280361.
  2. Xu J, Chi F, Guo T, Punj V, Lee WN, French SW, et al. NOTCH reprograms mitochondrial metabolism for proinflammatory macrophage activation. The Journal of clinical investigation. 2015;125(4):1579-90. doi: 10.1172/JCI76468. PubMed PMID: 25798621; PubMed Central PMCID: PMC4396469.
  3. Li Y, Wang J, Asahina K. Mesothelial cells give rise to hepatic stellate cells and myofibroblasts via mesothelial-mesenchymal transition in liver injury. Proceedings of the National Academy of Sciences of the United States of America. 2013;110(6):2324-9. doi: 10.1073/pnas.1214136110. PubMed PMID: 23345421; PubMed Central PMCID: PMC3568296.