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Anatoly V Grishin, PhD
Associate Professor of Research Surgery
CHL Mail Stop 65 Health Sciences Campus Los Angeles
+1 323 669 2104


Anatoly V. Grishin, PhD, a researcher with the Division of Pediatrics at Childrens Hospital Los Angeles and assistant professor of surgery at the Keck School of Medicine, received a two-year grant totaling $400,000 to fund a pilot study about how intestinal epithelial cells establish and maintain tolerance to Toll-like receptor (TLR) ligands of bacteria that populate the gastrointestinal tract.

The study will define roles of A20 and CYLD, the two key negative regulators of innate immune responses in intestinal tolerance to different TLR ligands.

Because intestinal inflammatory disorders such as necrotizing enterocolitis and IBD can be regarded as failure to establish and/or maintain tolerance to luminal microbiota, dissecting the mechanisms of intestinal tolerance to TLR ligands will lead to better understanding of these ailments, and, ultimately, better therapies.


Wang J, Grishin AV, Ford HR. Experimental Anti-Inflammatory Drug Semapimod Inhibits TLR Signaling by Targeting the TLR Chaperone gp96. J Immunol. 2016 Jun 15; 196(12):5130-7. View in: PubMed

Zagory JA, Nguyen MV, Dietz W, Mavila N, Haldeman A, Grishin A, Wang KS. Toll-like receptor 3 mediates PROMININ-1 expressing cell expansion in biliary atresia via Transforming Growth Factor-Beta. J Pediatr Surg. 2016 Jun; 51(6):917-22. View in: PubMed

Grishin A, Bowling J, Bell B, Wang J, Ford HR. Roles of nitric oxide and intestinal microbiota in the pathogenesis of necrotizing enterocolitis. J Pediatr Surg. 2016 Jan; 51(1):13-7. View in: PubMed

Short SS, Wang J, Castle SL, Fernandez GE, Smiley N, Zobel M, Pontarelli EM, Papillon SC, Grishin AV, Ford HR. Low doses of Celecoxib attenuate gut barrier failure during experimental peritonitis. Lab Invest. 2013 Dec; 93(12):1265-75. View in: PubMed

Grishin A, Papillon S, Bell B, Wang J, Ford HR. The role of the intestinal microbiota in the pathogenesis of necrotizing enterocolitis. Semin Pediatr Surg. 2013 May; 22(2):69-75. View in: PubMed

Jantscher-Krenn E, Zherebtsov M, Nissan C, Goth K, Guner YS, Naidu N, Choudhury B, Grishin AV, Ford HR, Bode L. The human milk oligosaccharide disialyllacto-N-tetraose prevents necrotising enterocolitis in neonatal rats. Gut. 2012 Oct; 61(10):1417-25. View in: PubMed

Emami CN, Chokshi N, Wang J, Hunter C, Guner Y, Goth K, Wang L, Grishin A, Ford HR. Role of interleukin-10 in the pathogenesis of necrotizing enterocolitis. Am J Surg. 2012 Apr; 203(4):428-35. View in: PubMed

McElroy SJ, Hobbs S, Kallen M, Tejera N, Rosen MJ, Grishin A, Matta P, Schneider C, Upperman J, Ford H, Polk DB, Weitkamp JH. Transactivation of EGFR by LPS Induces COX-2 Expression in Enterocytes. PLoS One. 2012; 7(5):e38373. View in: PubMed

Guner YS, Franklin AL, Chokshi NK, Castle SL, Pontarelli E, Wang J, Wang L, Prasadarao NV, Upperman JS, Grishin AV, Ford HR. P-glycoprotein induction by breast milk attenuates intestinal inflammation in experimental necrotizing enterocolitis. Lab Invest. 2011 Nov; 91(11):1668-79. View in: PubMed

Wang J, Ouyang Y, Guner Y, Ford HR, Grishin AV. Ubiquitin-editing enzyme A20 promotes tolerance to lipopolysaccharide in enterocytes. J Immunol. 2009 Jul 15; 183(2):1384-92. View in: PubMed

Guner YS, Ochoa CJ, Wang J, Zhang X, Steinhauser S, Stephenson L, Grishin A, Upperman JS. Peroxynitrite-induced p38 MAPK pro-apoptotic signaling in enterocytes. Biochem Biophys Res Commun. 2009 Jun 26; 384(2):221-5. View in: PubMed

Petrosyan M, Guner YS, Williams M, Grishin A, Ford HR. Current concepts regarding the pathogenesis of necrotizing enterocolitis. Pediatr Surg Int. 2009 Apr; 25(4):309-18. View in: PubMed

Tai CC, Curtis JL, Sala FG, Del Moral PM, Chokshi N, Kanard RJ, Al Alam D, Wang J, Burns RC, Ford HR, Grishin A, Wang KS, Bellusci S. Induction of fibroblast growth factor 10 (FGF10) in the ileal crypt epithelium after massive small bowel resection suggests a role for FGF10 in gut adaptation. Dev Dyn. 2009 Feb; 238(2):294-301. View in: PubMed

Hunter CJ, Singamsetty VK, Chokshi NK, Boyle P, Camerini V, Grishin AV, Upperman JS, Ford HR, Prasadarao NV. Enterobacter sakazakii enhances epithelial cell injury by inducing apoptosis in a rat model of necrotizing enterocolitis. J Infect Dis. 2008 Aug 15; 198(4):586-93. View in: PubMed

Chokshi NK, Guner YS, Hunter CJ, Upperman JS, Grishin A, Ford HR. The role of nitric oxide in intestinal epithelial injury and restitution in neonatal necrotizing enterocolitis. Semin Perinatol. 2008 Apr; 32(2):92-9. View in: PubMed

Lugo B, Ford HR, Grishin A. Molecular signaling in necrotizing enterocolitis: regulation of intestinal COX-2 expression. J Pediatr Surg. 2007 Jul; 42(7):1165-71. View in: PubMed

Grishin AV, Wang J, Potoka DA, Hackam DJ, Upperman JS, Boyle P, Zamora R, Ford HR. Lipopolysaccharide induces cyclooxygenase-2 in intestinal epithelium via a noncanonical p38 MAPK pathway. J Immunol. 2006 Jan 1; 176(1):580-8. View in: PubMed

Grishin A, Ford H, Wang J, Li H, Salvador-Recatala V, Levitan ES, Zaks-Makhina E. Attenuation of apoptosis in enterocytes by blockade of potassium channels. Am J Physiol Gastrointest Liver Physiol. 2005 Nov; 289(5):G815-21. View in: PubMed

Upperman JS, Potoka D, Grishin A, Hackam D, Zamora R, Ford HR. Mechanisms of nitric oxide-mediated intestinal barrier failure in necrotizing enterocolitis. Semin Pediatr Surg. 2005 Aug; 14(3):159-66. View in: PubMed

Qureshi FG, Leaphart C, Cetin S, Li J, Grishin A, Watkins S, Ford HR, Hackam DJ. Increased expression and function of integrins in enterocytes by endotoxin impairs epithelial restitution. Gastroenterology. 2005 Apr; 128(4):1012-22. View in: PubMed

Hackam DJ, Upperman JS, Grishin A, Ford HR. Disordered enterocyte signaling and intestinal barrier dysfunction in the pathogenesis of necrotizing enterocolitis. Semin Pediatr Surg. 2005 Feb; 14(1):49-57. View in: PubMed

Grishin A, Wang J, Hackam D, Qureshi F, Upperman J, Zamora R, Ford HR. p38 MAP kinase mediates endotoxin-induced expression of cyclooxygenase-2 in enterocytes. Surgery. 2004 Aug; 136(2):329-35. View in: PubMed

Potoka DA, Upperman JS, Zhang XR, Kaplan JR, Corey SJ, Grishin A, Zamora R, Ford HR. Peroxynitrite inhibits enterocyte proliferation and modulates Src kinase activity in vitro. Am J Physiol Gastrointest Liver Physiol. 2003 Nov; 285(5):G861-9. View in: PubMed

Wang L, Rudert WA, Grishin A, Dombrosky-Ferlan P, Sullivan K, Deng X, Whitcomb D, Corey S. Identification and genetic analysis of human and mouse activated Cdc42 interacting protein-4 isoforms. Biochem Biophys Res Commun. 2002 May 24; 293(5):1426-30. View in: PubMed

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