Faculty

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Guo-Min Li, PhD
Professor of Biochemistry & Molecular Biology
Biochemistry and Molecular Biology
NRT 3506 1450 Biggy Street Health Sciences Campus Los Angeles
+1 323 442 7436

Overview

Dr. Li is a professor in the Department of Biochemistry and Molecular Biology at the USC Keck School of Medicine. He received his bachelor’s degree from Wuhan University in China and his Ph.D. from Wayne State University. He performed his post-doctoral training with Nobel laureate Dr. Paul Modrich (Chemistry, 2015) at Duke University. Before joining the USC Norris Comprehensive Cancer Center in 2015, Dr. Li held professorship and the Madeline James and Edith Gardner Distinguished Chair in Cancer Research at the University of Kentucky Medical Center. Dr. Li is known for his seminal work in DNA mismatch repair (MMR). His major contributions to the field include discovering MMR defects in human cancer displaying microsatellite instability, identifying and characterizing key protein components required for MMR, reconstituting the human MMR reaction in vitro, and discovering the apoptotic function of MMR and regulation of human MMR by histone marks and posttranslational modifications. Dr. Li also studies the role of MMR in promoting (CAG)•(CTG) trinucleotide repeat expansion that causes many neurodegenerative disorders.

Publications

Li GM. A Personal Tribute to 2015 Nobel Laureate Paul Modrich. DNA Repair (Amst). 2016 Jan; 37:A14-21. View in: PubMed

Li GM. Celebrating the work of Nobel Laureate Paul Modrich. Sci China Life Sci. 2016 Jan; 59(1):93-6. View in: PubMed

Li GM. A Personal Tribute to 2015 Nobel Laureate Paul Modrich. DNA Repair (Amst). 2016 Jan; 37:A14-21. View in: PubMed

Hegde PM, Dutta A, Sengupta S, Mitra J, Adhikari S, Tomkinson AE, Li GM, Boldogh I, Hazra TK, Mitra S, Hegde ML. The C-terminal Domain (CTD) of Human DNA Glycosylase NEIL1 Is Required for Forming BERosome Repair Complex with DNA Replication Proteins at the Replicating Genome: DOMINANT NEGATIVE FUNCTION OF THE CTD. J Biol Chem. 2015 Aug 21; 290(34):20919-33. View in: PubMed

Sun L, Tan R, Xu J, LaFace J, Gao Y, Xiao Y, Attar M, Neumann C, Li GM, Su B, Liu Y, Nakajima S, Levine AS, Lan L. Targeted DNA damage at individual telomeres disrupts their integrity and triggers cell death. Nucleic Acids Res. 2015 Jul 27; 43(13):6334-47. View in: PubMed

Liu S, Lu G, Ali S, Liu W, Zheng L, Dai H, Li H, Xu H, Hua Y, Zhou Y, Ortega J, Li GM, Kunkel TA, Shen B. Okazaki fragment maturation involves a-segment error editing by the mammalian FEN1/MutSa functional complex. EMBO J. 2015 Jul 2; 34(13):1829-43. View in: PubMed

Tong D, Ortega J, Kim C, Huang J, Gu L, Li GM. Arsenic Inhibits DNA Mismatch Repair by Promoting EGFR Expression and PCNA Phosphorylation. J Biol Chem. 2015 Jun 5; 290(23):14536-41. View in: PubMed

Ortega J, Li JY, Lee S, Tong D, Gu L, Li GM. Phosphorylation of PCNA by EGFR inhibits mismatch repair and promotes misincorporation during DNA synthesis. Proc Natl Acad Sci U S A. 2015 May 5; 112(18):5667-72. View in: PubMed

He S, Zhao Z, Yang Y, O'Connell D, Zhang X, Oh S, Ma B, Lee JH, Zhang T, Varghese B, Yip J, Dolatshahi Pirooz S, Li M, Zhang Y, Li GM, Ellen Martin S, Machida K, Liang C. Truncating mutation in the autophagy gene UVRAG confers oncogenic properties and chemosensitivity in colorectal cancers. Nat Commun. 2015; 6:7839. View in: PubMed

Zhang M, Xiang S, Joo HY, Wang L, Williams KA, Liu W, Hu C, Tong D, Haakenson J, Wang C, Zhang S, Pavlovicz RE, Jones A, Schmidt KH, Tang J, Dong H, Shan B, Fang B, Radhakrishnan R, Glazer PM, Matthias P, Koomen J, Seto E, Bepler G, Nicosia SV, Chen J, Li C, Gu L, Li GM, Bai W, Wang H, Zhang X. HDAC6 deacetylates and ubiquitinates MSH2 to maintain proper levels of MutSa. Mol Cell. 2014 Jul 3; 55(1):31-46. View in: PubMed

Li GM. New insights and challenges in mismatch repair: getting over the chromatin hurdle. DNA Repair (Amst). 2014 Jul; 19:48-54. View in: PubMed

Li GM. Decoding the histone code: Role of H3K36me3 in mismatch repair and implications for cancer susceptibility and therapy. Cancer Res. 2013 Nov 1; 73(21):6379-83. View in: PubMed

Pinto RM, Dragileva E, Kirby A, Lloret A, Lopez E, St Claire J, Panigrahi GB, Hou C, Holloway K, Gillis T, Guide JR, Cohen PE, Li GM, Pearson CE, Daly MJ, Wheeler VC. Mismatch repair genes Mlh1 and Mlh3 modify CAG instability in Huntington's disease mice: genome-wide and candidate approaches. PLoS Genet. 2013 Oct; 9(10):e1003930. View in: PubMed

Hegde ML, Hegde PM, Bellot LJ, Mandal SM, Hazra TK, Li GM, Boldogh I, Tomkinson AE, Mitra S. Prereplicative repair of oxidized bases in the human genome is mediated by NEIL1 DNA glycosylase together with replication proteins. Proc Natl Acad Sci U S A. 2013 Aug 13; 110(33):E3090-9. View in: PubMed

Chan NL, Guo J, Zhang T, Mao G, Hou C, Yuan F, Huang J, Zhang Y, Wu J, Gu L, Li GM. Coordinated processing of 3' slipped (CAG)n/(CTG)n hairpins by DNA polymerases ß and d preferentially induces repeat expansions. J Biol Chem. 2013 May 24; 288(21):15015-22. View in: PubMed

Li F, Mao G, Tong D, Huang J, Gu L, Yang W, Li GM. The histone mark H3K36me3 regulates human DNA mismatch repair through its interaction with MutSa. Cell. 2013 Apr 25; 153(3):590-600. View in: PubMed

Stevens JR, Lahue EE, Li GM, Lahue RS. Trinucleotide repeat expansions catalyzed by human cell-free extracts. Cell Res. 2013 Apr; 23(4):565-72. View in: PubMed

Chen X, Zhao Y, Li GM, Guo L. Proteomic analysis of mismatch repair-mediated alkylating agent-induced DNA damage response. Cell Biosci. 2013; 3(1):37. View in: PubMed

Martín-López JV, Barrios Y, Medina-Arana V, Andújar M, Lee S, Gu L, Li GM, Rüschoff J, Salido E, Fishel R. The hMSH2(M688R) Lynch syndrome mutation may function as a dominant negative. Carcinogenesis. 2012 Sep; 33(9):1647-54. View in: PubMed

Chan NL, Hou C, Zhang T, Yuan F, Machwe A, Huang J, Orren DK, Gu L, Li GM. The Werner syndrome protein promotes CAG/CTG repeat stability by resolving large (CAG)(n)/(CTG)(n) hairpins. J Biol Chem. 2012 Aug 31; 287(36):30151-6. View in: PubMed

Mao G, Lee S, Ortega J, Gu L, Li GM. Modulation of microRNA processing by mismatch repair protein MutLa. Cell Res. 2012 Jun; 22(6):973-85. View in: PubMed

Wang HT, Hu Y, Tong D, Huang J, Gu L, Wu XR, Chung FL, Li GM, Tang MS. Effect of carcinogenic acrolein on DNA repair and mutagenic susceptibility. J Biol Chem. 2012 Apr 6; 287(15):12379-86. View in: PubMed

Zhang T, Huang J, Gu L, Li GM. In vitro repair of DNA hairpins containing various numbers of CAG/CTG trinucleotide repeats. DNA Repair (Amst). 2012 Feb 1; 11(2):201-9. View in: PubMed

Li GM. A special issue on DNA damage response and genome stability. Cell Biosci. 2012; 2:4. View in: PubMed

Gu L, Ensor CM, Li GM. In vitro DNA mismatch repair in human cells. Methods Mol Biol. 2012; 920:135-47. View in: PubMed

Machwe A, Lozada E, Wold MS, Li GM, Orren DK. Molecular cooperation between the Werner syndrome protein and replication protein A in relation to replication fork blockage. J Biol Chem. 2011 Feb 4; 286(5):3497-508. View in: PubMed

Hou C, Zhang T, Tian L, Huang J, Gu L, Li GM. The Role of XPG in Processing (CAG)n/(CTG)n DNA Hairpins. Cell Biosci. 2011; 1(1):11. View in: PubMed

Li GM. Novel molecular insights into the mechanism of GO removal by MutM. Cell Res. 2010 Feb; 20(2):116-8. View in: PubMed

Li F, Tian L, Gu L, Li GM. Evidence that nucleosomes inhibit mismatch repair in eukaryotic cells. J Biol Chem. 2009 Nov 27; 284(48):33056-61. View in: PubMed

Hou C, Chan NL, Gu L, Li GM. Incision-dependent and error-free repair of (CAG)(n)/(CTG)(n) hairpins in human cell extracts. Nat Struct Mol Biol. 2009 Aug; 16(8):869-75. View in: PubMed

Tian L, Hou C, Tian K, Holcomb NC, Gu L, Li GM. Mismatch recognition protein MutSbeta does not hijack (CAG)n hairpin repair in vitro. J Biol Chem. 2009 Jul 31; 284(31):20452-6. View in: PubMed

Tian L, Gu L, Li GM. Distinct nucleotide binding/hydrolysis properties and molar ratio of MutSalpha and MutSbeta determine their differential mismatch binding activities. J Biol Chem. 2009 Apr 24; 284(17):11557-62. View in: PubMed

Cannella M, Maglione V, Martino T, Ragona G, Frati L, Li GM, Squitieri F. DNA instability in replicating Huntington's disease lymphoblasts. BMC Med Genet. 2009; 10:11. View in: PubMed

Shao C, Xiong S, Li GM, Gu L, Mao G, Markesbery WR, Lovell MA. Altered 8-oxoguanine glycosylase in mild cognitive impairment and late-stage Alzheimer's disease brain. Free Radic Biol Med. 2008 Sep 15; 45(6):813-9. View in: PubMed

Zhang Y, Yuan F, Wang D, Gu L, Li GM. Identification of regulatory factor X as a novel mismatch repair stimulatory factor. J Biol Chem. 2008 May 9; 283(19):12730-5. View in: PubMed

Li GM. Mechanisms and functions of DNA mismatch repair. Cell Res. 2008 Jan; 18(1):85-98. View in: PubMed

Mao G, Pan X, Zhu BB, Zhang Y, Yuan F, Huang J, Lovell MA, Lee MP, Markesbery WR, Li GM, Gu L. Identification and characterization of OGG1 mutations in patients with Alzheimer's disease. Nucleic Acids Res. 2007; 35(8):2759-66. View in: PubMed

Guo S, Zhang Y, Yuan F, Gao Y, Gu L, Wong I, Li GM. Regulation of replication protein A functions in DNA mismatch repair by phosphorylation. J Biol Chem. 2006 Aug 4; 281(31):21607-16. View in: PubMed

Gu L, Li GM. Analysis of DNA mismatch repair in cellular response to DNA damage. Methods Enzymol. 2006; 408:303-17. View in: PubMed

Zhang Y, Yuan F, Presnell SR, Tian K, Gao Y, Tomkinson AE, Gu L, Li GM. Reconstitution of 5'-directed human mismatch repair in a purified system. Cell. 2005 Sep 9; 122(5):693-705. View in: PubMed

Murata H, Khattar NH, Gu L, Li GM. Roles of mismatch repair proteins hMSH2 and hMLH1 in the development of sporadic breast cancer. Cancer Lett. 2005 Jun 1; 223(1):143-50. View in: PubMed

Vo AT, Zhu F, Wu X, Yuan F, Gao Y, Gu L, Li GM, Lee TH, Her C. hMRE11 deficiency leads to microsatellite instability and defective DNA mismatch repair. EMBO Rep. 2005 May; 6(5):438-44. View in: PubMed

Yuan F, Gu L, Guo S, Wang C, Li GM. Evidence for involvement of HMGB1 protein in human DNA mismatch repair. J Biol Chem. 2004 May 14; 279(20):20935-40. View in: PubMed

Guo S, Presnell SR, Yuan F, Zhang Y, Gu L, Li GM. Differential requirement for proliferating cell nuclear antigen in 5' and 3' nick-directed excision in human mismatch repair. J Biol Chem. 2004 Apr 23; 279(17):16912-7. View in: PubMed

McCulloch SD, Gu L, Li GM. Nick-dependent and -independent processing of large DNA loops in human cells. J Biol Chem. 2003 Dec 12; 278(50):50803-9. View in: PubMed

Wu J, Zhu BB, Yu J, Zhu H, Qiu L, Kindy MS, Gu L, Seidel A, Li GM. In vitro and in vivo modulations of benzo[c]phenanthrene-DNA adducts by DNA mismatch repair system. Nucleic Acids Res. 2003 Nov 15; 31(22):6428-34. View in: PubMed

Li GM, Presnell SR, Gu L. Folate deficiency, mismatch repair-dependent apoptosis, and human disease. J Nutr Biochem. 2003 Oct; 14(10):568-75. View in: PubMed

Li GM. DNA mismatch repair and cancer. Front Biosci. 2003 May 1; 8:d997-1017. View in: PubMed

McCulloch SD, Gu L, Li GM. Bi-directional processing of DNA loops by mismatch repair-dependent and -independent pathways in human cells. J Biol Chem. 2003 Feb 7; 278(6):3891-6. View in: PubMed

Murata H, Khattar NH, Kang Y, Gu L, Li GM. Genetic and epigenetic modification of mismatch repair genes hMSH2 and hMLH1 in sporadic breast cancer with microsatellite instability. Oncogene. 2002 Aug 22; 21(37):5696-703. View in: PubMed

Gu L, Cline-Brown B, Zhang F, Qiu L, Li GM. Mismatch repair deficiency in hematological malignancies with microsatellite instability. Oncogene. 2002 Aug 22; 21(37):5758-64. View in: PubMed

Gu L, Wu J, Zhu BB, Li GM. Deficiency of a novel mismatch repair activity in a bladder tumor cell line. Nucleic Acids Res. 2002 Jul 1; 30(13):2758-63. View in: PubMed

Gu L, Wu J, Qiu L, Jennings CD, Li GM. Involvement of DNA mismatch repair in folate deficiency-induced apoptosis small star, filled. J Nutr Biochem. 2002 Jun; 13(6):355-363. View in: PubMed

Ramilo C, Gu L, Guo S, Zhang X, Patrick SM, Turchi JJ, Li GM. Partial reconstitution of human DNA mismatch repair in vitro: characterization of the role of human replication protein A. Mol Cell Biol. 2002 Apr; 22(7):2037-46. View in: PubMed

Wu J, Gu L, Wang H, Geacintov NE, Li GM. Mismatch repair processing of carcinogen-DNA adducts triggers apoptosis. Mol Cell Biol. 1999 Dec; 19(12):8292-301. View in: PubMed

Wang H, Lawrence CW, Li GM, Hays JB. Specific binding of human MSH2. MSH6 mismatch-repair protein heterodimers to DNA incorporating thymine- or uracil-containing UV light photoproducts opposite mismatched bases. J Biol Chem. 1999 Jun 11; 274(24):16894-900. View in: PubMed

Li GM. The role of mismatch repair in DNA damage-induced apoptosis. Oncol Res. 1999; 11(9):393-400. View in: PubMed

Veigl ML, Kasturi L, Olechnowicz J, Ma AH, Lutterbaugh JD, Periyasamy S, Li GM, Drummond J, Modrich PL, Sedwick WD, Markowitz SD. Biallelic inactivation of hMLH1 by epigenetic gene silencing, a novel mechanism causing human MSI cancers. Proc Natl Acad Sci U S A. 1998 Jul 21; 95(15):8698-702. View in: PubMed

Eshleman JR, Donover PS, Littman SJ, Swinler SE, Li GM, Lutterbaugh JD, Willson JK, Modrich P, Sedwick WD, Markowitz SD, Veigl ML. Increased transversions in a novel mutator colon cancer cell line. Oncogene. 1998 Mar 5; 16(9):1125-30. View in: PubMed

Gu L, Hong Y, McCulloch S, Watanabe H, Li GM. ATP-dependent interaction of human mismatch repair proteins and dual role of PCNA in mismatch repair. Nucleic Acids Res. 1998 Mar 1; 26(5):1173-8. View in: PubMed

Li GM, Wang H, Romano LJ. Human MutSalpha specifically binds to DNA containing aminofluorene and acetylaminofluorene adducts. J Biol Chem. 1996 Sep 27; 271(39):24084-8. View in: PubMed

Eshleman JR, Markowitz SD, Donover PS, Lang EZ, Lutterbaugh JD, Li GM, Longley M, Modrich P, Veigl ML, Sedwick WD. Diverse hypermutability of multiple expressed sequence motifs present in a cancer with microsatellite instability. Oncogene. 1996 Apr 4; 12(7):1425-32. View in: PubMed

Drummond JT, Li GM, Longley MJ, Modrich P. Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells. Science. 1995 Jun 30; 268(5219):1909-12. View in: PubMed

Parsons R, Li GM, Longley M, Modrich P, Liu B, Berk T, Hamilton SR, Kinzler KW, Vogelstein B. Mismatch repair deficiency in phenotypically normal human cells. Science. 1995 May 5; 268(5211):738-40. View in: PubMed

Li GM, Modrich P. Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs. Proc Natl Acad Sci U S A. 1995 Mar 14; 92(6):1950-4. View in: PubMed

Parsons R, Li GM, Longley MJ, Fang WH, Papadopoulos N, Jen J, de la Chapelle A, Kinzler KW, Vogelstein B, Modrich P. Hypermutability and mismatch repair deficiency in RER+ tumor cells. Cell. 1993 Dec 17; 75(6):1227-36. View in: PubMed

Kat A, Thilly WG, Fang WH, Longley MJ, Li GM, Modrich P. An alkylation-tolerant, mutator human cell line is deficient in strand-specific mismatch repair. Proc Natl Acad Sci U S A. 1993 Jul 15; 90(14):6424-8. View in: PubMed

Fang WH, Li GM, Longley M, Holmes J, Thilly W, Modrich P. Mismatch repair and genetic stability in human cells. Cold Spring Harb Symp Quant Biol. 1993; 58:597-603. View in: PubMed

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