Faculty

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Michael E. Selsted, MD, PhD
Chair and Professor of Pathology
Pathology
HMR 204 Health Sciences Campus Los Angeles
+1 323 442 1180

Overview

Dr. Selsted is an internationally renowned scholar in the field of innate immunity and pathogenesis whose research discoveries have resulted in more than 20 U.S. patents. In addition to his pioneering research and clinical science, Dr. Selsted is a prolific author and experienced administrator. Under his leadership, National Institutes of Health and total research funding at the University of California, Irvine Department of Pathology and Laboratory Medicine increased more than four-fold to $6.6 million.

Publications

Rhesus ?-defensin-1 (RTD-1) exhibits in vitro and in vivo activity against cystic fibrosis strains of Pseudomonas aeruginosa. J Antimicrob Chemother. 2016 Jan; 71(1):181-8. View in: PubMed

Rhesus macaque ?-defensin RTD-1 inhibits proinflammatory cytokine secretion and gene expression by inhibiting the activation of NF-?B and MAPK pathways. Rhesus macaque? B and MAPK pathways. J Leukoc Biol. 2015 Dec; 98(6):1061-70. View in: PubMed

Microbicidal effects of a- and ?-defensins against antibiotic-resistant Staphylococcus aureus and Pseudomonas aeruginosa. Innate Immun. 2015 Jan; 21(1):17-29. View in: PubMed

Hydrophobic Determinants of a-Defensin Bactericidal Activity. Infect Immun. 2014 Jun; 82(6):2195-202. View in: PubMed

Killing of staphylococci by ?-defensins involves membrane impairment and activation of autolytic enzymes. Antibiotics (Basel). 2014; 3(4):617-31. View in: PubMed

Differential Susceptibility of Bacteria to Mouse Paneth Cell a-Defensins under Anaerobic Conditions. Antibiotics (Basel). 2014; 3(4):493-508. View in: PubMed

Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature. Nat Prod Rep. 2013 Jan; 30(1):108-60. View in: PubMed

Immunology. HD6 defensin nanonets. Science. 2012 Jul 27; 337(6093):420-1. View in: PubMed

Alternative Luminal Activation Mechanisms for Paneth Cell a-Defensins. J Biol Chem. 2012 Mar 30; 287(14):11205-12. View in: PubMed

RTD-1Mimic Containing ?PNA Scaffold Exhibits Broad-Spectrum Antibacterial Activities. J Am Chem Soc. 2012 Mar 7; 134(9):4041-4. View in: PubMed

Rhesus macaque theta defensins suppress inflammatory cytokines and enhance survival in mouse models of bacteremic sepsis. PLoS One. 2012; 7(12):e51337. View in: PubMed

High Fidelity Processing and Activation of the Human a-Defensin HNP1 Precursor by Neutrophil Elastase and Proteinase 3. PLoS One. 2012; 7(3):e32469. View in: PubMed

Cycloquest: identification of cyclopeptides via database search of their mass spectra against genome databases. J Proteome Res. 2011 Oct 7; 10(10):4505-12. View in: PubMed

Criterion for amino acid composition of defensins and antimicrobial peptides based on geometry of membrane destabilization. J Am Chem Soc. 2011 May 4; 133(17):6720-7. View in: PubMed

Rhesus macaque ?-defensin isoforms: expression, antimicrobial activities, and demonstration of a prominent role in neutrophil granule microbicidal activities. J Leukoc Biol. 2011 Feb; 89(2):283-90. View in: PubMed

Cyclic and acyclic defensins inhibit human immunodeficiency virus type-1 replication by different mechanisms. PLoS One. 2010; 5(3):e9737. View in: PubMed

Rhesus theta-defensin prevents death in a mouse model of severe acute respiratory syndrome coronavirus pulmonary disease. J Virol. 2009 Nov; 83(21):11385-90. View in: PubMed

SDF2L1, a component of the endoplasmic reticulum chaperone complex, differentially interacts with {alpha}-, {beta}-, and {theta}-defensin propeptides. J Biol Chem. 2009 Feb 27; 284(9):5602-9. View in: PubMed

The cell-penetrating peptide, Pep-1, has activity against intracellular chlamydial growth but not extracellular forms of Chlamydia trachomatis. J Antimicrob Chemother. 2009 Jan; 63(1):115-23. View in: PubMed

Synthesis, structure, and activities of an oral mucosal alpha-defensin from rhesus macaque. J Biol Chem. 2008 Dec 19; 283(51):35869-77. View in: PubMed

Isolation, synthesis, and antimicrobial activities of naturally occurring theta-defensin isoforms from baboon leukocytes. Infect Immun. 2008 Dec; 76(12):5883-91. View in: PubMed

Microbicidal properties and cytocidal selectivity of rhesus macaque theta defensins. Antimicrob Agents Chemother. 2008 Mar; 52(3):944-53. View in: PubMed

A pocket guide to explorations of the defensin field. Curr Pharm Des. 2007; 13(30):3061-4. View in: PubMed

Bovine peptidoglycan recognition protein-S: antimicrobial activity, localization, secretion, and binding properties. J Immunol. 2006 Jan 15; 176(2):1154-62. View in: PubMed

Mammalian defensins in the antimicrobial immune response. Nat Immunol. 2005 Jun; 6(6):551-7. View in: PubMed

Theta-defensins: cyclic antimicrobial peptides produced by binary ligation of truncated alpha-defensins. Curr Protein Pept Sci. 2004 Oct; 5(5):365-71. View in: PubMed

Structure-activity determinants in paneth cell alpha-defensins: loss-of-function in mouse cryptdin-4 by charge-reversal at arginine residue positions. J Biol Chem. 2004 Mar 19; 279(12):11976-83. View in: PubMed

Paneth cell alpha-defensins from rhesus macaque small intestine. Infect Immun. 2004 Mar; 72(3):1470-8. View in: PubMed

Alpha-defensin expression during myelopoiesis: identification of cis and trans elements that regulate expression of NP-3 in rat promyelocytes. J Leukoc Biol. 2004 Feb; 75(2):332-41. View in: PubMed

Quantitative interactions between cryptdin-4 amino terminal variants and membranes. Peptides. 2003 Nov; 24(11):1795-805. View in: PubMed

alpha-Defensins can have anti-HIV activity but are not CD8 cell anti-HIV factors. AIDS. 2003 Sep 26; 17(14):F23-32. View in: PubMed

Susceptibility of human and murine Chlamydia trachomatis serovars to granulocyte- and epithelium-derived antimicrobial peptides. J Pept Res. 2003 May; 61(5):237-42. View in: PubMed

Antimicrobial properties of the Escherichia coli R1 plasmid host killing peptide. J Biotechnol. 2003 Jan 9; 100(1):1-12. View in: PubMed

Antimicrobial peptides from human platelets. Infect Immun. 2002 Dec; 70(12):6524-33. View in: PubMed

Isolation, characterization, and antimicrobial properties of bovine oligosaccharide-binding protein. A microbicidal granule protein of eosinophils and neutrophils. J Biol Chem. 2002 May 31; 277(22):19658-64. View in: PubMed

Cryptdin 3 forms anion selective channels in cytoplasmic membranes of human embryonic kidney cells. Am J Physiol Gastrointest Liver Physiol. 2002 May; 282(5):G757-65. View in: PubMed

Homodimeric theta-defensins from rhesus macaque leukocytes: isolation, synthesis, antimicrobial activities, and bacterial binding properties of the cyclic peptides. J Biol Chem. 2002 Feb 1; 277(5):3079-84. View in: PubMed

Cryptdin-3 induces novel apical conductance(s) in Cl- secretory, including cystic fibrosis, epithelia. Am J Physiol Cell Physiol. 2001 Feb; 280(2):C296-302. View in: PubMed

Characterization of luminal paneth cell alpha-defensins in mouse small intestine. Attenuated antimicrobial activities of peptides with truncated amino termini. J Biol Chem. 2000 Oct 27; 275(43):33969-73. View in: PubMed

Identification of constituents of human neutrophil azurophil granules that mediate fungistasis against Histoplasma capsulatum. Infect Immun. 2000 Oct; 68(10):5668-72. View in: PubMed

Secretion of microbicidal alpha-defensins by intestinal Paneth cells in response to bacteria. Nat Immunol. 2000 Aug; 1(2):113-8. View in: PubMed

Formation and characterization of a single Trp-Trp cross-link in indolicidin that confers protease stability without altering antimicrobial activity. J Biol Chem. 2000 Apr 21; 275(16):12017-22. View in: PubMed

Peptide localization and gene structure of cryptdin 4, a differentially expressed mouse paneth cell alpha-defensin. Infect Immun. 1999 Dec; 67(12):6643-51. View in: PubMed

Isolation, characterization, cDNA cloning, and antimicrobial properties of two distinct subfamilies of alpha-defensins from rhesus macaque leukocytes. Infect Immun. 1999 Nov; 67(11):6139-44. View in: PubMed

A cyclic antimicrobial peptide produced in primate leukocytes by the ligation of two truncated alpha-defensins. Science. 1999 Oct 15; 286(5439):498-502. View in: PubMed

CD spectra of indolicidin antimicrobial peptides suggest turns, not polyproline helix. Biochemistry. 1999 Sep 21; 38(38):12313-9. View in: PubMed

Cloning and expression of bovine neutrophil beta-defensins. Biosynthetic profile during neutrophilic maturation and localization of mature peptide to novel cytoplasmic dense granules. J Biol Chem. 1999 Sep 10; 274(37):26249-58. View in: PubMed

Anti-HIV-1 activity of indolicidin, an antimicrobial peptide from neutrophils. J Leukoc Biol. 1998 Jan; 63(1):94-100. View in: PubMed

The t(8;21) fusion product, AML-1-ETO, associates with C/EBP-alpha, inhibits C/EBP-alpha-dependent transcription, and blocks granulocytic differentiation. Mol Cell Biol. 1998 Jan; 18(1):322-33. View in: PubMed

Semidry electroblotting of peptides and proteins from acid-urea polyacrylamide gels. Anal Biochem. 1997 Nov 15; 253(2):225-30. View in: PubMed

Critical role of lipid composition in membrane permeabilization by rabbit neutrophil defensins. J Biol Chem. 1997 Sep 26; 272(39):24224-33. View in: PubMed

Induction of epithelial chloride secretion by channel-forming cryptdins 2 and 3. Proc Natl Acad Sci U S A. 1997 Aug 5; 94(16):8585-9. View in: PubMed

Purification and in vitro activities of rabbit platelet microbicidal proteins. Infect Immun. 1997 Mar; 65(3):1023-31. View in: PubMed

Cryptdin gene expression in developing mouse small intestine. Am J Physiol. 1997 Jan; 272(1 Pt 1):G197-206. View in: PubMed

HPLC methods for purification of antimicrobial peptides. Methods Mol Biol. 1997; 78:17-33. View in: PubMed

Interactions of monomeric rabbit neutrophil defensins with bilayers: comparison with dimeric human defensin HNP-2. Biochemistry. 1996 Sep 10; 35(36):11888-94. View in: PubMed

Paneth cell defensins: endogenous peptide components of intestinal host defense. FASEB J. 1996 Sep; 10(11):1280-9. View in: PubMed

Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils. J Biol Chem. 1996 Jul 5; 271(27):16430. View in: PubMed

Rat neutrophil defensins. Precursor structures and expression during neutrophilic myelopoiesis. J Immunol. 1995 Nov 1; 155(9):4476-84. View in: PubMed

Solution structure of bovine neutrophil beta-defensin-12: the peptide fold of the beta-defensins is identical to that of the classical defensins. Biochemistry. 1995 Oct 17; 34(41):13663-71. View in: PubMed

Structure, function, and membrane integration of defensins. Curr Opin Struct Biol. 1995 Aug; 5(4):521-7. View in: PubMed

Synthesis and characterization of indolicidin, a tryptophan-rich antimicrobial peptide from bovine neutrophils. Int J Pept Protein Res. 1995 May; 45(5):401-9. View in: PubMed

Defensins in granules of phagocytic and non-phagocytic cells. Trends Cell Biol. 1995 Mar; 5(3):114-9. View in: PubMed

Killing of Giardia lamblia by cryptdins and cationic neutrophil peptides. Infect Immun. 1994 Dec; 62(12):5397-403. View in: PubMed

Mouse Paneth cell defensins: primary structures and antibacterial activities of numerous cryptdin isoforms. Infect Immun. 1994 Nov; 62(11):5040-7. View in: PubMed

Structure and dynamics of the neutrophil defensins NP-2, NP-5, and HNP-1: NMR studies of amide hydrogen exchange kinetics. Proteins. 1994 Sep; 20(1):52-67. View in: PubMed

Interactions between human defensins and lipid bilayers: evidence for formation of multimeric pores. Protein Sci. 1994 Sep; 3(9):1362-73. View in: PubMed

Structure and diversity of the murine cryptdin gene family. Genomics. 1994 Feb; 19(3):448-53. View in: PubMed

Fungicidal properties of defensin NP-1 and activity against Cryptococcus neoformans in vitro. Antimicrob Agents Chemother. 1993 Dec; 37(12):2628-32. View in: PubMed

Defensins promote fusion and lysis of negatively charged membranes. Protein Sci. 1993 Aug; 2(8):1301-12. View in: PubMed

Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils. J Biol Chem. 1993 Mar 25; 268(9):6641-8. View in: PubMed

Characterization of the disulfide motif in BNBD-12, an antimicrobial beta-defensin peptide from bovine neutrophils. J Biol Chem. 1993 Mar 25; 268(9):6649-53. View in: PubMed

Investigational approaches for studying the structures and biological functions of myeloid antimicrobial peptides. Genet Eng (N Y). 1993; 15:131-47. View in: PubMed

NMR studies of defensin antimicrobial peptides. 1. Resonance assignment and secondary structure determination of rabbit NP-2 and human HNP-1. Biochemistry. 1992 Nov 24; 31(46):11348-56. View in: PubMed

NMR studies of defensin antimicrobial peptides. 2. Three-dimensional structures of rabbit NP-2 and human HNP-1. Biochemistry. 1992 Nov 24; 31(46):11357-64. View in: PubMed

Enteric defensins: antibiotic peptide components of intestinal host defense. J Cell Biol. 1992 Aug; 118(4):929-36. View in: PubMed

Purification and primary structure of murine cryptdin-1, a Paneth cell defensin. FEBS Lett. 1992 Jun 15; 304(2-3):146-8. View in: PubMed

Indolicidin, a novel bactericidal tridecapeptide amide from neutrophils. J Biol Chem. 1992 Mar 5; 267(7):4292-5. View in: PubMed

Antimicrobial activity of rabbit leukocyte defensins against Treponema pallidum subsp. pallidum. Infect Immun. 1991 Apr; 59(4):1359-67. View in: PubMed

Crystal structure of defensin HNP-3, an amphiphilic dimer: mechanisms of membrane permeabilization. Science. 1991 Mar 22; 251(5000):1481-5. View in: PubMed

Defensins: endogenous antibiotic peptides of animal cells. Cell. 1991 Jan 25; 64(2):229-30. View in: PubMed

Killing of oral, gram-negative, facultative bacteria by the rabbit defensin, NP-1. Oral Microbiol Immunol. 1990 Dec; 5(6):315-9. View in: PubMed

In vitro sensitivity of oral, gram-negative, facultative bacteria to the bactericidal activity of human neutrophil defensins. Infect Immun. 1990 Dec; 58(12):3934-40. View in: PubMed

Characterization of defensin resistance phenotypes associated with mutations in the phoP virulence regulon of Salmonella typhimurium. Infect Immun. 1990 Nov; 58(11):3706-10. View in: PubMed

In vitro antimicrobial activity of defensins against ocular pathogens. Arch Ophthalmol. 1990 Jun; 108(6):861-4. View in: PubMed

Defensins. Eur J Haematol. 1990 Jan; 44(1):1-8. View in: PubMed

Antimicrobial defensin peptides form voltage-dependent ion-permeable channels in planar lipid bilayer membranes. Proc Natl Acad Sci U S A. 1990 Jan; 87(1):210-4. View in: PubMed

Monocyte-chemotactic activity of defensins from human neutrophils. J Clin Invest. 1989 Dec; 84(6):2017-20. View in: PubMed

Interaction of human defensins with Escherichia coli. Mechanism of bactericidal activity. J Clin Invest. 1989 Aug; 84(2):553-61. View in: PubMed

Purification and antimicrobial properties of three defensins from rat neutrophils. Infect Immun. 1989 Jul; 57(7):2021-7. View in: PubMed

Evidence for a pretranslational defect in hereditary and acquired myeloperoxidase deficiency. Blood. 1989 May 15; 73(7):1980-6. View in: PubMed

Determination of the disulfide array in the human defensin HNP-2. A covalently cyclized peptide. J Biol Chem. 1989 Mar 5; 264(7):4003-7. View in: PubMed

Neutrophils and host defense. Ann Intern Med. 1988 Jul 15; 109(2):127-42. View in: PubMed

Solution structures of the rabbit neutrophil defensin NP-5. J Mol Biol. 1988 Jun 5; 201(3):625-36. View in: PubMed

Modulation of the in vitro candidacidal activity of human neutrophil defensins by target cell metabolism and divalent cations. J Clin Invest. 1988 Jun; 81(6):1829-35. View in: PubMed

Synergistic cytolysis mediated by hydrogen peroxide combined with peptide defensins. Cell Immunol. 1988 Jun; 114(1):104-16. View in: PubMed

Characterization of two crystal forms of human defensin neutrophil cationic peptide 1, a naturally occurring antimicrobial peptide of leukocytes. J Biol Chem. 1988 Apr 25; 263(12):5933-5. View in: PubMed

Mechanism of target cytolysis by peptide defensins. Target cell metabolic activities, possibly involving endocytosis, are crucial for expression of cytotoxicity. J Immunol. 1988 Apr 15; 140(8):2686-94. View in: PubMed

Inhibition of protein kinase C by defensins, antibiotic peptides from human neutrophils. Biochem Pharmacol. 1988 Mar 1; 37(5):951-6. View in: PubMed

Oxygen-independent bactericidal systems. Mechanisms and disorders. Hematol Oncol Clin North Am. 1988 Mar; 2(1):159-69. View in: PubMed

Purification, primary structure, and antimicrobial activities of a guinea pig neutrophil defensin. Infect Immun. 1987 Sep; 55(9):2281-6. View in: PubMed

Defensin-rich dense granules of human neutrophils. Blood. 1987 Sep; 70(3):757-65. View in: PubMed

In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes. Blood. 1986 Dec; 68(6):1407-10. View in: PubMed

Direct inactivation of viruses by human granulocyte defensins. J Virol. 1986 Dec; 60(3):1068-74. View in: PubMed

In vitro killing of spores and hyphae of Aspergillus fumigatus and Rhizopus oryzae by rabbit neutrophil cationic peptides and bronchoalveolar macrophages. J Infect Dis. 1986 Sep; 154(3):483-9. View in: PubMed

Synergistic activity of rabbit granulocyte peptides against Candida albicans. Infect Immun. 1986 Jun; 52(3):902-4. View in: PubMed

Antimicrobial activity of phagocyte granule proteins. Semin Respir Infect. 1986 Jun; 1(2):107-17. View in: PubMed

Defensins. Natural peptide antibiotics of human neutrophils. J Clin Invest. 1985 Oct; 76(4):1427-35. View in: PubMed

Newborn rabbit alveolar macrophages are deficient in two microbicidal cationic peptides, MCP-1 and MCP-2. Am Rev Respir Dis. 1985 Oct; 132(4):901-4. View in: PubMed

Primary structures of three human neutrophil defensins. J Clin Invest. 1985 Oct; 76(4):1436-9. View in: PubMed

Correlation of binding of rabbit granulocyte peptides to Candida albicans with candidacidal activity. Infect Immun. 1985 Jul; 49(1):207-11. View in: PubMed

Activity of rabbit leukocyte peptides against Candida albicans. Infect Immun. 1985 Jul; 49(1):202-6. View in: PubMed

In vitro effect of phagocyte cationic peptides on Coccidioides immitis. J Infect Dis. 1985 May; 151(5):890-4. View in: PubMed

Direct inactivation of viruses by MCP-1 and MCP-2, natural peptide antibiotics from rabbit leukocytes. J Virol. 1985 May; 54(2):467-72. View in: PubMed

Opsonic activity of MCP-1 and MCP-2, cationic peptides from rabbit alveolar macrophages. Diagn Microbiol Infect Dis. 1985 May; 3(3):233-42. View in: PubMed

Primary structures of six antimicrobial peptides of rabbit peritoneal neutrophils. J Biol Chem. 1985 Apr 25; 260(8):4579-84. View in: PubMed

Divergent expression of cytotoxic and microbicidal functions of rabbit alveolar and peritoneal macrophages: effects of non-specific activation and a natural microbicidal peptide, MCP-1. Aust J Exp Biol Med Sci. 1985 Feb; 63 ( Pt 1):53-63. View in: PubMed

Characterization of two crystal forms of neutrophil cationic protein NP2, a naturally occurring broad-spectrum antimicrobial agent from leukocytes. J Mol Biol. 1984 Sep 25; 178(3):783-5. View in: PubMed

Purification and antibacterial activity of antimicrobial peptides of rabbit granulocytes. Infect Immun. 1984 Jul; 45(1):150-4. View in: PubMed

Primary structures of MCP-1 and MCP-2, natural peptide antibiotics of rabbit lung macrophages. J Biol Chem. 1983 Dec 10; 258(23):14485-9. View in: PubMed

Antibacterial activity of microbicidal cationic proteins 1 and 2, natural peptide antibiotics of rabbit lung macrophages. Infect Immun. 1983 Oct; 42(1):10-4. View in: PubMed

Increased content of microbicidal cationic peptides in rabbit alveolar macrophages elicited by complete Freund adjuvant. Infect Immun. 1981 Sep; 33(3):775-8. View in: PubMed

The theta-defensin RTD-1 improves insulin action and normalizes plasma glucose and FFA levels in diet-induced obese rats. Am J Physiol Endocrinol Metab. 2015 May 19; ajpendo. 00131. 2015. View in: PubMed

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