Faculty

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Raymond D Mosteller, PhD
Associate Professor of Biochemistry & Molecular Medicine
Director of The Computer Curriculum, Educational Affairs
Medicine
BMT B11 E 1333 San Pablo Street Health Sciences Campus Los Angeles
+1 323 442 1588

Overview

CURRENT RESEARCH INTERESTS:
Dr. Mosteller's research interests are in the area of guanine nucleotide binding proteins (G-proteins) including the Ras proteins (e.g. k-ras) from eucaryotic organisms including yeast (Saccharomyces cerevisiae) and humans. His most recent research has involved studies of the interactions of G-proteins with other regulatory molecules in the cell such as the guanine nucleotide exchange factor (GNEF) and Vav. He is also interested in the membrane phosphoinositides, PIP2 and PIP3, and the PI-3-kinase signalling pathway.

PREVIOUS RESEARCH INTERESTS:
His previous research interests include the following: regulation of expression of the tryptophan operon in Escherichia coli; interaction of the alpha and beta subunits of tryptophan synthase from Escherichia coli; protein metabolism and turnover in Escherichia coli; interaction of transducin protein with rhodopsin in photoreceptor cells; the mechanism of protein synthesis in vitro on polyribosomes from rabbit reticulocytes.

EDUCATION AND TRAINING:
Dr. Mosteller received his B.A. and Ph.D. degrees from the University of Texas in Austin. As an undergraduate, he was elected into the Phi Eta Sigma and Phi Beta Kappa honor societies. His Ph.D. work was with Dr. Boyd Hardesty studying protein synthesis in vitro on polyribosomes from rabbit reticulocytes. He completed his postdoctoral studies at Stanford University working with Dr. Charles Yanofsky on the regulation of expression of the tryptophan operon in Escherichia coli. Dr. Mosteller also spent a one year sabbatical with Dr. Melvin Simon at the California Institute of Technology (Caltech) studying the interaction of the protein transducin with rhodopsin in photoreceptor cells. After his sabbatical, Dr. Mosteller collaborated for 10 years with Dr. Daniel Broek in the USC Norris Comprehensive Cancer Center studying various aspects of Ras and G-protein regulation in yeast (Saccharomyces cerevisiae) and humans.

Publications

Sphingosine kinase protects lipopolysaccharide-activated macrophages from apoptosis. Mol Cell Biol. 2004 Sep; 24(17):7359-69. View in: PubMed

VEGF receptor expression and signaling in human bladder tumors. Oncogene. 2003 May 29; 22(22):3361-70. View in: PubMed

Sphingosine kinase mediates vascular endothelial growth factor-induced activation of ras and mitogen-activated protein kinases. Mol Cell Biol. 2002 Nov; 22(22):7758-68. View in: PubMed

Biochemical analysis of regulation of Vav, a guanine-nucleotide exchange factor for Rho family of GTPases. Methods Enzymol. 2000; 325:38-51. View in: PubMed

Distinct subclasses of small GTPases interact with guanine nucleotide exchange factors in a similar manner. Mol Cell Biol. 1998 Dec; 18(12):7444-54. View in: PubMed

Role of substrates and products of PI 3-kinase in regulating activation of Rac-related guanosine triphosphatases by Vav. Science. 1998 Jan 23; 279(5350):558-60. View in: PubMed

Lck regulates Vav activation of members of the Rho family of GTPases. Mol Cell Biol. 1997 Mar; 17(3):1346-53. View in: PubMed

Identification of a dominant-negative mutation in the yeast CDC25 guanine nucleotide exchange factor for Ras. Oncogene. 1997 Feb 20; 14(7):831-6. View in: PubMed

Involvement of the switch 2 domain of Ras in its interaction with guanine nucleotide exchange factors. J Biol Chem. 1996 May 10; 271(19):11076-82. View in: PubMed

Analysis of interaction between Ras and CDC25 guanine nucleotide exchange factor using yeast GAL4 two-hybrid system. Methods Enzymol. 1995; 255:135-48. View in: PubMed

Amino acid residues in the CDC25 guanine nucleotide exchange factor critical for interaction with Ras. Mol Cell Biol. 1994 Dec; 14(12):8117-22. View in: PubMed

Identification of residues of the H-ras protein critical for functional interaction with guanine nucleotide exchange factors. Mol Cell Biol. 1994 Feb; 14(2):1104-12. View in: PubMed

Identification of a mammalian gene structurally and functionally related to the CDC25 gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1992 Aug 1; 89(15):7100-4. View in: PubMed

Improved method for measuring the catalytic activity of tryptophan synthase alpha subunit in cell extracts. Biochimie. 1990 Dec; 72(12):881-4. View in: PubMed

A mathematical model that applies to protein degradation and post-translational processing of proteins and to analogous processes for other molecules in non-growing and exponentially growing cells. J Theor Biol. 1984 Jun 21; 108(4):597-621. View in: PubMed

Post-translational modification of a tryptophan biosynthetic enzyme. Precursor-product relationships in vivo and carbon-energy source dependence. J Biol Chem. 1983 Nov 10; 258(21):12793-6. View in: PubMed

Energy-dependent inactivation and modification of a tryptophan biosynthetic enzyme in Escherichia coli. J Biol Chem. 1982 Sep 10; 257(17):10184-90. View in: PubMed

Metabolism of individual proteins in exponentially growing Escherichia coli. J Biol Chem. 1980 Mar 25; 255(6):2524-32. View in: PubMed

Evidence that glucose starvation-sensitive mutants are altered in the relB locus. J Bacteriol. 1978 Feb; 133(2):1034-7. View in: PubMed

Genetic and segregation analysis of Escherichia coli strains containing a tandem duplication of the trpD-purB region of the chromosome. J Bacteriol. 1978 Feb; 133(2):650-60. View in: PubMed

Role of methionine in the synthesis of nucleoside Q in Escherichia coli transfer ribonucleic acid. J Bacteriol. 1977 Oct; 132(1):174-9. View in: PubMed

Interactions of tryptophan synthetase subunits in Escherichia coli containing mutationally altered beta2 subunits. J Biol Chem. 1977 Jul 10; 252(13):4527-32. View in: PubMed

Inactivation and partial degradation of phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase in nongrowing cultures of Escherichia coli. J Bacteriol. 1977 Jul; 131(1):153-62. View in: PubMed

Isolation of relaxed-control mutants of escherichia coli K-12 which are sensitive to glucose starvation. Biochem Biophys Res Commun. 1976 Mar 22; 69(2):325-32. View in: PubMed

Inhibition of nucleoside Q formation in transfer ribonucleic acid during methionine starvation of relaxed-control Escherichia coli. J Bacteriol. 1976 Jan; 125(1):205-10. View in: PubMed

Increased loss of duplicated genes in streptomycin-resistant (strA) mutants of Escherichia coli k-12. J Bacteriol. 1976 Jan; 125(1):382-4. View in: PubMed

Unusual sensitivity of Escherichia coli to adenine or adenine plus histidine. J Bacteriol. 1975 Aug; 123(2):750-1. View in: PubMed

Kinetics of derepression of the tryptophan operon of Escherichia coli and Salmonella typhimurium under different culture conditions. J Mol Biol. 1973 Nov 15; 80(4):801-23. View in: PubMed

Evidence that tryptophanyl transfer ribonucleic acid is not the corepressor of the tryptophan operon of Escherichia coli. J Bacteriol. 1971 Jan; 105(1):268-75. View in: PubMed

Tryptophan messenger ribonucleic acid elongation rates and steady-state levels of tryptophan operon enzymes under various growth conditions. J Mol Biol. 1970 Aug; 51(3):541-50. View in: PubMed

Transcription of the tryptophan operon in Escherichia coli: rifampicin as an inhibitor of initiation. J Mol Biol. 1970 Mar; 48(3):525-31. View in: PubMed

Direction of in vivo degradation of tryptophan messenger RNA--a correction. Nature. 1969 Jul 5; 223(5201):40-3. View in: PubMed

Dynamics of synthesis, translation, and degradation of trp operon messenger RNA in E. coli. Cold Spring Harb Symp Quant Biol. 1969; 34:725-40. View in: PubMed

Deacylated transfer ribonucleic acid as a factor for peptide chain initiation in rabbit reticulocyte systems. J Biol Chem. 1968 Dec 25; 243(24):6343-52. View in: PubMed

Involvement of transfer ribonucleic acid in early reactions of polyphenylalanine synthesis. Arch Biochem Biophys. 1968 May; 125(2):658-70. View in: PubMed

The requirement for tRNA for the shift in the optimum Mg++ concentration during the synthesis of polyphenylalanine. Biochem Biophys Res Commun. 1968 Mar 27; 30(6):631-6. View in: PubMed

A reaction associated with nonenzymatic binding in the reticulocyte transfer system. Proc Natl Acad Sci U S A. 1967 Jun; 57(6):1817-24. View in: PubMed

The mechanism of sodium fluoride and cycloheximide inhibition of hemoglobin biosynthesis in the cell-free reticulocyte system. J Mol Biol. 1966 Oct 28; 21(1):51-69. View in: PubMed

Differential inactivation of soluble reticulocyte transfer factors with N-ethylmaleimide. Biochem Biophys Res Commun. 1966 Sep 8; 24(5):714-9. View in: PubMed

NaF inhibition of the initial binding of aminoacyl-sRNA to reticulocyte ribosomes. Proc Natl Acad Sci U S A. 1966 Aug; 56(2):701-8. View in: PubMed

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