Faculty

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Stanley Tahara, PhD
Associate Professor of Molecular Microbiology & Immunology
Molecular Microbiology and Immunology
HMR 510A 2011 Zonal Avenue Health Sciences Campus Los Angeles
+1 323 442 1722

Overview

Our laboratory is interested in post-transcriptional regulation. We are currently examining the biological significance of protein arginine methylation of translation initiation factors. We are currently studying the effect of Arg362 methylation of factor eIF4A. The latter is the prototype of DEXD/H box nucleotide dependent helicases. We have found that methylation of Arg362 results in: 1) significantly reduced ATPase activity and 2) inhibition of eIF4A assembly with eIF4G into the eIF4F heterotrimer. We believe this example of covalent modification is representative of a previously unknown regulatory circuit for modulating cellular protein synthesis. Our current efforts are directed towards identifying regulators of protein methylation and further examination of eIF4A with respect to mRNA specific effects in protein synthesis.

Publications

Li C, Zhou Y, Loberg A, Tahara SM, Malik P, Kalra VK. Activated Transcription Factor 3 in Association with Histone Deacetylase 6 Negatively Regulates MicroRNA 199a2 Transcription by Chromatin Remodeling and Reduces Endothelin-1 Expression. Mol Cell Biol. 2016 Nov 15; 36(22):2838-2854. View in: PubMed

Chen CL, Uthaya Kumar DB, Punj V, Xu J, Sher L, Tahara SM, Hess S, Machida K. NANOG Metabolically Reprograms Tumor-Initiating Stem-like Cells through Tumorigenic Changes in Oxidative Phosphorylation and Fatty Acid Metabolism. Cell Metab. 2016 Jan 12; 23(1):206-19. View in: PubMed

Gonsalves CS, Li C, Mpollo MS, Pullarkat V, Malik P, Tahara SM, Kalra VK. Erythropoietin-mediated expression of placenta growth factor is regulated via activation of hypoxia-inducible factor-1a and post-transcriptionally by miR-214 in sickle cell disease. Biochem J. 2015 Jun 15; 468(3):409-23. View in: PubMed

Li C, Gonsalves CS, Eiymo Mwa Mpollo MS, Malik P, Tahara SM, Kalra VK. MicroRNA 648 Targets ET-1 mRNA and Is Cotranscriptionally Regulated with MICAL3 by PAX5. Mol Cell Biol. 2015 Feb 1; 35(3):514-28. View in: PubMed

Gonsalves CS, Li C, Malik P, Tahara SM, Kalra VK. Peroxisome proliferator-activated receptor-a-mediated transcription of miR-301a and miR-454 and their host gene SKA2 regulates endothelin-1 and PAI-1 expression in sickle cell disease. Biosci Rep. 2015; 35(6). View in: PubMed

Li C, Mpollo MS, Gonsalves CS, Tahara SM, Malik P, Kalra VK. Peroxisome Proliferator-activated Receptor-a-mediated Transcription of miR-199a2 Attenuates Endothelin-1 Expression via Hypoxia-inducible Factor-1a. J Biol Chem. 2014 Dec 26; 289(52):36031-47. View in: PubMed

Ferreira R, Santos T, Amar A, Tahara SM, Chen TC, Giannotta SL, Hofman FM. MicroRNA-18a Improves Human Cerebral Arteriovenous Malformation Endothelial Cell Function. Stroke. 2014 Jan; 45(1):293-7. View in: PubMed

Ferreira R, Santos T, Amar A, Gong A, Chen TC, Tahara SM, Giannotta SL, Hofman FM. Argonaute-2 Promotes miR-18a Entry in Human Brain Endothelial Cells. J Am Heart Assoc. 2014; 3(3). View in: PubMed

Pullarkat V, Meng Z, Tahara SM, Johnson CS, Kalra VK. Proteasome inhibition induces both antioxidant and hb f responses in sickle cell disease via the nrf2 pathway. Hemoglobin. 2014; 38(3):188-95. View in: PubMed

Pham LK, Liang M, Adisetiyo HA, Liao CP, Cohen MB, Tahara SM, Frenkel B, Kasahara N, Roy-Burman P. Contextual effect of repression of bone morphogenetic protein activity in prostate cancer. Endocr Relat Cancer. 2013 Dec; 20(6):861-74. View in: PubMed

Patel N, Tahara SM, Malik P, Kalra VK. Involvement of miR-30c and miR-301a in immediate induction of plasminogen activator inhibitor-1 by placental growth factor in human pulmonary endothelial cells. Biochem J. 2011 Mar 15; 434(3):473-82. View in: PubMed

Wooderchak WL, Zang T, Zhou ZS, Acuña M, Tahara SM, Hevel JM. Substrate profiling of PRMT1 reveals amino acid sequences that extend beyond the "RGG" paradigm. Biochemistry. 2008 Sep 9; 47(36):9456-66. View in: PubMed

Hsu YC, Acuña M, Tahara SM, Peng CA. Reduced phagocytosis of colloidal carriers using soluble CD47. Pharm Res. 2003 Oct; 20(10):1539-42. View in: PubMed

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