Dr. Grikscheit, a member of The Saban Research Institute's Developmental Biology and Regenerative Medicine program, envisions a better solution. The small intestine is an exquisitely regenerative organ. It is an elegant model to better understand organ-specific stem cells and the regenerative cells can be harnessed to make new gastrointestinal tissue for eventual clinical applications. Understanding how to generate tissue-engineered intestines, whether esophagus, stomach, small intestine, or colon, may also inform us how to expand the list of organs that can be tissue-engineered.
Tissue-Engineering the Intestine: The Trials before the Trials Cell Stem Cell. 2019 Jun 06; 24(6):855-859. . View in PubMed
Wnt signaling inhibition by monensin results in a period of Hippo pathway activation during intestinal adaptation in zebrafish Am J Physiol Gastrointest Liver Physiol. 2019 Jun 01; 316(6):G679-G691. . View in PubMed
Differential epithelial growth in tissue-engineered larynx and trachea generated from postnatal and fetal progenitor cells Biochem Biophys Res Commun. 2019 Mar 05; 510(2):205-210. . View in PubMed
Stem cells for babies and their surgeons: The future is now J Pediatr Surg. 2019 Jan; 54(1):16-20. . View in PubMed
Short-term and long-term human or mouse organoid units generate tissue-engineered small intestine without added signalling molecules Exp Physiol. 2018 Dec; 103(12):1633-1644. . View in PubMed
Stem cells for babies and their surgeons: the future is now J Pediatr Surg. 2018 Oct 05. . View in PubMed
Short-term and long-term human or mouse organoid units generate tissue-engineered small intestine without added signaling molecules Exp Physiol. 2018 Sep 19. . View in PubMed
Corrigendum to "Inhibition of Fgf signaling in short bowel syndrome increases weight loss and epithelial proliferation" [surgery volume 161, number 3 (2017) 694-703]. Surgery. 2018 Feb 13. . View in PubMed
Short-term and long-term human or mouse organoid units generate tissue-engineered small intestine without added signalling molecules Exp Physiol. 2018 12; 103(12):1633-1644. . View in PubMed
Corrigendum to "Inhibition of Fgf signaling in short bowel syndrome increases weight loss and epithelial proliferation" [surgery volume 161, number 3 (2017) 694-703]. Surgery. 2018 06; 163(6):1330-1331. . View in PubMed
Marked stem/progenitor cell expansion occurs early after murine ileostomy: a new model J Surg Res. 2017 Dec; 220:182-196. . View in PubMed
Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells Cold Spring Harb Perspect Med. 2017 Oct 03; 7(10). . View in PubMed
Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine Stem Cell Reports. 2017 Sep 12; 9(3):883-896. . View in PubMed
Prolonged Absence of Mechanoluminal Stimulation in Human Intestine Alters the Transcriptome and Intestinal Stem Cell NicheCell Mol Gastroenterol Hepatol. 2017 May; 3(3):367-388. e1. . View in PubMed
Marked stem/progenitor cell expansion occurs early after murine ileostomy: a new model J Surg Res. 2017 12; 220:182-196. . View in PubMed
Intestinal adaptation in proximal and distal segments: Two epithelial responses diverge after intestinal separation Surgery. 2017 04; 161(4):1016-1027. . View in PubMed
Fibroblast Growth Factors in the Gastrointestinal Tract: Twists and Turns Dev Dyn. 2017 04; 246(4):344-352. . View in PubMed
Inhibition of Fgf signaling in short bowel syndrome increases weight loss and epithelial proliferation Surgery. 2017 03; 161(3):694-703. . View in PubMed
Short bowel syndrome results in increased gene expression associated with proliferation, inflammation, bile acid synthesis and immune system activation: RNA sequencing a zebrafish SBS model BMC Genomics. 2017 01 25; 18(1):23. . View in PubMed
Functional Human and Murine Tissue-Engineered Liver Is Generated from Adult Stem/Progenitor Cells Stem Cells Transl Med. 2017 Jan; 6(1):238-248. . View in PubMed
Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine Stem Cell Reports. 2017 09 12; 9(3):883-896. . View in PubMed
Created of Warm Blood and Nerves: Restoring an Enteric Nervous System in Organoids Cell Stem Cell. 2017 01 05; 20(1):5-7. . View in PubMed
Deriving human ENS lineages for cell therapy and drug discovery in Hirschsprung disease Nature. 2016 Mar 03; 531(7592):105-9. . View in PubMed
Fgf10 overexpression enhances the formation of tissue-engineered small intestine J Tissue Eng Regen Med. 2016 Feb; 10(2):132-9. . View in PubMed
Establishing Proximal and Distal Regional Identities in Murine and Human Tissue-Engineered Lung and Trachea Tissue Eng Part C Methods. 2016 11; 22(11):1049-1057. . View in PubMed
The pediatric intestinal mucosal microbiome remains altered after clinical resolution of inflammatory and ischemic disease Surgery. 2016 08; 160(2):350-8. . View in PubMed
Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic Tissue Eng Part A. 2016 Jan; 22(1-2):53-64. . View in PubMed
Sequestration of Vascular Endothelial Growth Factor (VEGF) Induces Late Restrictive Lung Disease PLoS One. 2016; 11(2):e0148323. . View in PubMed
Vascular Endothelial Growth Factor (VEGF) Bioavailability Regulates Angiogenesis and Intestinal Stem and Progenitor Cell Proliferation during Postnatal Small Intestinal Development PLoS One. 2016; 11(3):e0151396. . View in PubMed
Generation of tissue-engineered small intestine using embryonic stem cell-derived human intestinal organoids Biol Open. 2015 Oct 12; 4(11):1462-72. . View in PubMed
Adult zebrafish intestine resection: a novel model of short bowel syndrome, adaptation, and intestinal stem cell regeneration Am J Physiol Gastrointest Liver Physiol. 2015 Aug 01; 309(3):G135-45. . View in PubMed
Human and mouse tissue-engineered small intestine both demonstrate digestive and absorptive function Am J Physiol Gastrointest Liver Physiol. 2015 Apr 15; 308(8):G664-77. . View in PubMed
Fibroblast growth factor 10 alters the balance between goblet and Paneth cells in the adult mouse small intestine Am J Physiol Gastrointest Liver Physiol. 2015 Apr 15; 308(8):G678-90. . View in PubMed
Murine and human tissue-engineered esophagus form from sufficient stem/progenitor cells and do not require microdesigned biomaterials Tissue Eng Part A. 2015 Mar; 21(5-6):906-15. . View in PubMed
Gremlin 1 identifies a skeletal stem cell with bone, cartilage, and reticular stromal potential Cell. 2015 Jan 15; 160(1-2):269-84. . View in PubMed
Vitrification preserves murine and human donor cells for generation of tissue-engineered intestine J Surg Res. 2014 Aug; 190(2):399-406. . View in PubMed
Enteric nervous system cell replacement therapy for Hirschsprung disease: beyond tissue-engineered intestine Eur J Pediatr Surg. 2014 Jun; 24(3):214-8. . View in PubMed
Regenerative medicine: is this the biggest change in surgery? Semin Pediatr Surg. 2014 Jun; 23(3):103-5.. View in PubMed
Stem cells and biopharmaceuticals: vital roles in the growth of tissue-engineered small intestine Semin Pediatr Surg. 2014 Jun; 23(3):141-9. . View in PubMed
Delayed family reunification of pediatric disaster survivors increases mortality and inpatient hospital costs: a simulation study J Surg Res. 2013 Sep; 184(1):430-7. . View in PubMed
Tissue engineering: a promising therapeutic approach to necrotizing enterocolitis Semin Pediatr Surg. 2013 May; 22(2):112-6. . View in PubMed
Tissue engineering the small intestine Clin Gastroenterol Hepatol. 2013 Apr; 11(4):354-8. . View in PubMed
Human tissue-engineered small intestine forms from postnatal progenitor cells J Pediatr Surg. 2013 Jan; 48(1):129-37. . View in PubMed
Forearm skeletal muscle neuroblastoma in a child: a rare primary location J Pediatr Hematol Oncol. 2013 Jan; 35(1):61-3. . View in PubMed
A "living bioreactor" for the production of tissue-engineered small intestine Methods Mol Biol. 2013; 1001:299-309. . View in PubMed
Tissue engineering of the intestine in a murine model J Vis Exp. 2012 Dec 01; (70):e4279. . View in PubMed
Intraoperative hypercyanosis in a patient with pulmonary artery band: case report and review of the literature J Clin Anesth. 2012 Dec; 24(8):652-5. . View in PubMed
Human tissue-engineered colon forms from postnatal progenitor cells: an in vivo murine model Regen Med. 2012 Nov; 7(6):807-18. . View in PubMed
FGF9-Pitx2-FGF10 signaling controls cecal formation in mice Dev Biol. 2012 Sep 15; 369(2):340-8. . View in PubMed
Giant cystic meconium peritonitis presenting in a neonate with classic radiographic eggshell calcifications and treated with an elective surgical approach: a case report J Med Case Rep. 2012 Aug 02; 6:229. . View in PubMed
Solid pseudopapillary tumor of the pancreas: a single-institution 20-year series of pediatric patients J Pediatr Surg. 2012 Jun; 47(6):1217-22. . View in PubMed
Tissue-engineering of the gastrointestinal tract Curr Opin Pediatr. 2012 Jun; 24(3):365-70. . View in PubMed
Fibroblast growth factor 10-fibroblast growth factor receptor 2b mediated signaling is not required for adult glandular stomach homeostasis PLoS One. 2012; 7(11):e49127. . View in PubMed
Treatment of congenital pulmonary airway malformation induced hydrops fetalis via percutaneous sclerotherapy Fetal Diagn Ther. 2012; 31(4):264-8. . View in PubMed
Mesenchymal-specific inhibition of vascular endothelial growth factor (VEGF) attenuates growth in neonatal mice J Surg Res. 2012 Jan; 172(1):40-7. . View in PubMed
Tissue-engineered small intestine Expert Rev Med Devices. 2011 Nov; 8(6):673-5. . View in PubMed
Murine tissue-engineered stomach demonstrates epithelial differentiation J Surg Res. 2011 Nov; 171(1):6-14. . View in PubMed
Availability of a pediatric trauma center in a disaster surge decreases triage time of the pediatric surge population: a population kinetics model Theor Biol Med Model. 2011 Oct 12; 8:38. . View in PubMed
VEGF optimizes the formation of tissue-engineered small intestine Regen Med. 2011 Sep; 6(5):559-67. . View in PubMed
A multicellular approach forms a significant amount of tissue-engineered small intestine in the mouse Tissue Eng Part A. 2011 Jul; 17(13-14):1841-50. . View in PubMed
FGF10 controls the patterning of the tracheal cartilage rings via Shh Development. 2011 Jan; 138(2):273-82. . View in PubMed
Massive subcutaneous emphysema, pneumomediastinum, and pneumopericardium in children J Pediatr Surg. 2010 Mar; 45(3):647-9. . View in PubMed
Wnt5a knock-out mouse as a new model of anorectal malformation J Surg Res. 2009 Oct; 156(2):278-82. . View in PubMed
Tissue-engineered small intestine and stomach form from autologous tissue in a preclinical large animal model J Surg Res. 2009 Oct; 156(2):205-12. . View in PubMed
Thoracoscopic repair of neonatal diaphragmatic hernia J Laparoendosc Adv Surg Tech A. 2008 Dec; 18(6):875-80. . View in PubMed
Preservation of extracorporeal tissue in closing gastroschisis augments intestinal length J Pediatr Surg. 2008 Dec; 43(12):2213-5. . View in PubMed
Necrotizing enterocolitis--bench to bedside: novel and emerging strategies Semin Pediatr Surg. 2008 Nov; 17(4):255-65. . View in PubMed
Tissue-engineered spleen protects against overwhelming pneumococcal sepsis in a rodent model J Surg Res. 2008 Oct; 149(2):214-8. . View in PubMed
Cost considerations and applicant characteristics for the Pediatric Surgery Match J Pediatr Surg. 2005 Jan; 40(1):69-73; discussion 73-4. . View in PubMed
Tissue-engineered small intestine improves recovery after massive small bowel resection Ann Surg. 2004 Nov; 240(5):748-54. . View in PubMed
Lymphangiogenesis in tissue-engineered small intestine Transplantation. 2004 Apr 27; 77(8):1162-6. . View in PubMed
Effect of GLP-2 on mucosal morphology and SGLT1 expression in tissue-engineered neointestine Am J Physiol Gastrointest Liver Physiol. 2003 Dec; 285(6):G1345-52. . View in PubMed
Angiogenesis in tissue-engineered small intestine Tissue Eng. 2003 Dec; 9(6):1255-61. . View in PubMed
Tissue-engineered stomach: a preliminary report of a versatile in vivo model with therapeutic potential J Pediatr Surg. 2003 Sep; 38(9):1305-9. . View in PubMed
Tissue engineering of the gastrointestinal tract for surgical replacement: a nutrition tool of the future? Proc Nutr Soc. 2003 Aug; 62(3):739-43.. View in PubMed
Tissue-engineered esophagus: experimental substitution by onlay patch or interposition J Thorac Cardiovasc Surg. 2003 Aug; 126(2):537-44. . View in PubMed
Tissue-engineered large intestine resembles native colon with appropriate in vitro physiology and architecture Ann Surg. 2003 Jul; 238(1):35-41. . View in PubMed
Tissue-engineered small intestine: ontogeny of the immune system Transplantation. 2002 Sep 15; 74(5):619-23. . View in PubMed
Tissue-engineered colon exhibits function in vivo Surgery. 2002 Aug; 132(2):200-4. . View in PubMed
The history and current status of tissue engineering: The future of pediatric surgery J Pediatr Surg. 2002 Mar; 37(3):277-88. . View in PubMed
A novel tumor-derived mediator that sensitizes cytokine-resistant tumors to tumor necrosis factor J Surg Res. 1996 Jun; 63(1):248-55. . View in PubMed
Characterization of a novel tumor-derived cytokineEndothelial-monocyte activating polypeptide II. J Biol Chem. 1994 Oct 07; 269(40):25106-19. . View in PubMed
