Keck School Faculty

Ya-Wen Chen
Ya-Wen Chen
Assistant Professor of Medicine
Medicine
2020 Zonal Avenue Off Campus Los Angeles
Currently, the only way to replace lung tissue is to perform lung transplantation, which is an complicate procedure that achieves only 10 to 20% survival at 10 years and is hampered by a severe shortage of donor organs. The ability to reconstruct lung tissue from hPSCs would fundamentally change the outlook of pulmonary medicine. In vitro generated lung tissue could be used for regenerative purposes. Alternatively, postnatal lung stem cells could be generated in sufficient quantities from hPSCs and used for stem-cell therapies and regenerative medicine. Over the past few years, lung progenitor populations have been identified in mouse models. It has become clear that many types of differentiated epithelial cells in the lung, such as club and ATII cells, can function as stem cells in steady state and following injury. Our research interests are applying human pluripotent stem cells (hPSCs, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)) as a model to study the mechanisms of lung injury repair and ultimately apply the hPSC-derived lung stem/progenitor or mature airway/lung epithelial cells to facilitate lung injury repair, stem-cell therapy, and regenerative medicine.

Francis Family Foundation: Parker B. Francis Fellowship, 2018

International Society for Stem Cell Research: Travel Award, 2017

NHLBI Progenitor Cell Biology Consortium: Training Award-human pluripotent stem cell biology and gene editing, 2017

NHLBI Progenitor Cell Biology Consortium: Training Award-Multiplexed in situ hybridzation, 2017

Columbia University Medical Center: Samuel Bard Young Investigator Awards, 2015

International Society for Stem Cell Research: Travel Award, 2015

Journal of Investigative Dermatology: Profile December: Meet the Investigator, 2014

Gordon Research Conference: 2nd place, Student Category of Plasminogen Activation & Extracellular Proteolysis , 2010

University of Maryland, Baltimore: Travel Award, 2010

: Winner of Biochemical Journal Poster Prize, 2010

Gordon Research Conference: Winner of Biochemical Journal Poster Prize, 2010

: Masterpiece of Biology of National Science & Experiment Competition, 1999

Taiwan: Masterpiece of Biology of National Science & Experiment Competition, 1999

: Young Scientist Award of the 1st APEC Youth Science Festival, 1998

Asia-Pacific Economic Cooperation (APEC): Young Scientist Award of the 1st APEC Youth Science Festival, 1998

Taiwan: The third Award of the 38th National Private and high schools Science Fair, 1998

: The third Award of the 38th National Private and high schools Science Fair, 1998

Glycogen synthase kinase 3 induces multilineage maturation of human pluripotent stem cell-derived lung progenitors in 3D culture Development. 2019 Jan 22; 146(2). . View in PubMed

New insights and therapeutic targets: Lung injury and disease J Thorac Cardiovasc Surg. 2019 Jan; 157(1):416-420. . View in PubMed

3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch SignalingCell Stem Cell. 2018 Oct 04; 23(4):516-529. e5. . View in PubMed

Teratogenic Effects of Topiramate in a Zebrafish Model Int J Mol Sci. 2017 Aug 07; 18(8). . View in PubMed

Functional vascularized lung grafts for lung bioengineering Sci Adv. 2017 Aug; 3(8):e1700521. . View in PubMed

A three-dimensional model of human lung development and disease from pluripotent stem cells Nat Cell Biol. 2017 May; 19(5):542-549. . View in PubMed

Functional vascularized lung grafts for lung bioengineering Sci Adv. 2017 08; 3(8):e1700521. . View in PubMed

Generation of three-dimensional lung bud organoid and its derived branching colonies Protocol Exchange. 2017. . View in PubMed

Cross-circulation for extracorporeal support and recovery of the lung Nature Biomedical Engineering. 2017. . View in PubMed

N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability Int J Mol Sci. 2016 Jun 14; 17(6). . View in PubMed

Plasminogen-Dependent Matriptase Activation Accelerates Plasmin Generation by Differentiating Primary Human Keratinocytes J Invest Dermatol. 2016 06; 136(6):1210-1218. . View in PubMed

The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells Nat Protoc. 2015 Mar; 10(3):413-25. . View in PubMed

Hypoxia-induced retinal neovascularization in zebrafish embryos: a potential model of retinopathy of prematurity PLoS One. 2015; 10(5):e0126750. . View in PubMed

Matriptase regulates proliferation and early, but not terminal, differentiation of human keratinocytes J Invest Dermatol. 2014 Feb; 134(2):405-414. . View in PubMed

Efficient generation of lung and airway epithelial cells from human pluripotent stem cells Nat Biotechnol. 2014 Jan; 32(1):84-91. . View in PubMed

Matriptase expression and zymogen activation in human pilosebaceous unit J Histochem Cytochem. 2014 Jan; 62(1):50-9. . View in PubMed

Imbalanced matriptase pericellular proteolysis contributes to the pathogenesis of malignant B-cell lymphomas Am J Pathol. 2013 Oct; 183(4):1306-17. . View in PubMed

Amikacin-induced Fin Reduction is Mediated by Autophagy J Toxicol Pathol. 2013 Mar; 26(1):79-82. . View in PubMed

Antithrombin regulates matriptase activity involved in plasmin generation, syndecan shedding, and HGF activation in keratinocytes PLoS One. 2013; 8(5):e62826. . View in PubMed

Mechanisms for the control of matriptase activity in the absence of sufficient HAI-1 Am J Physiol Cell Physiol. 2012 Jan 15; 302(2):C453-62. . View in PubMed

Analgesic and Antiinflammatory Activities of the Aqueous Extract from Plectranthus amboinicus (Lour) Spreng. Both In Vitro and In Vivo. Evid Based Complement Alternat Med. 2012; 2012:508137. . View in PubMed

The transmembrane serine protease matriptase: implications for cellular biology and human diseases J Med Sci. 2012. . View in PubMed

Handbook of Proteolytic Enzymes (Third edition)MS. 681|Matriptase. 2012. . View in PubMed

Cardiac myosin binding protein C and MAP-kinase activating death domain-containing gene polymorphisms and diastolic heart failure PLoS One. 2012; 7(4):e35242. . View in PubMed

Essential roles of basic helix-loop-helix transcription factors, Capsulin and Musculin, during craniofacial myogenesis of zebrafish Cell Mol Life Sci. 2011 Dec; 68(24):4065-78. . View in PubMed

Targeting zymogen activation to control the matriptase-prostasin proteolytic cascade J Med Chem. 2011 Nov 10; 54(21):7567-78. . View in PubMed

Matriptase is inhibited by extravascular antithrombin in epithelial cells but not in most carcinoma cells Am J Physiol Cell Physiol. 2011 Nov; 301(5):C1093-103. . View in PubMed

Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli J Inorg Biochem. 2011 Jun; 105(6):902-10. . View in PubMed

Increased matriptase zymogen activation in inflammatory skin disorders Am J Physiol Cell Physiol. 2011 Mar; 300(3):C406-15. . View in PubMed

Biochemical and structural properties of zebrafish Capsulin produced by Escherichia coli Protein Expr Purif. 2011 Jan; 75(1):21-7. . View in PubMed

Type I transglutaminase accumulation in the endoplasmic reticulum may be an underlying cause of autosomal recessive congenital ichthyosis J Biol Chem. 2010 Oct 08; 285(41):31634-46. . View in PubMed

Regulation of the matriptase-prostasin cell surface proteolytic cascade by hepatocyte growth factor activator inhibitor-1 during epidermal differentiation J Biol Chem. 2010 Oct 08; 285(41):31755-62. . View in PubMed

TMPRSS2, a serine protease expressed in the prostate on the apical surface of luminal epithelial cells and released into semen in prostasomes, is misregulated in prostate cancer cells Am J Pathol. 2010 Jun; 176(6):2986-96. . View in PubMed

Outer membrane protein OmpF involved in the transportation of polypyridyl ruthenium complexes into Escherichia coli J Inorg Biochem. 2010 May; 104(5):614-7. . View in PubMed

Transgenic zebrafish line with over-expression of Hedgehog on the skin: a useful tool to screen Hedgehog-inhibiting compounds Transgenic Res. 2009 Dec; 18(6):855-64. . View in PubMed

A novel phenotype-based approach for systematically screening antiproliferation metallodrugs Chem Biol Interact. 2009 Nov 10; 182(1):84-91. . View in PubMed

Polarized epithelial cells secrete matriptase as a consequence of zymogen activation and HAI-1-mediated inhibition Am J Physiol Cell Physiol. 2009 Aug; 297(2):C459-70. . View in PubMed

Movement disorder and neuromuscular change in zebrafish embryos after exposure to caffeine Neurotoxicol Teratol. 2008 Sep-Oct; 30(5):440-7. . View in PubMed

Zymogen activation, inhibition, and ectodomain shedding of matriptase Front Biosci. 2008 Jan 01; 13:621-35. . View in PubMed

Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish Dev Biol. 2006 Nov 15; 299(2):594-608. . View in PubMed

A keratin 18 transgenic zebrafish Tg(k18(29):RFP) treated with inorganic arsenite reveals visible overproliferation of epithelial cells. Toxicol Lett. 2006 Jun 01; 163(3):191-7. . View in PubMed

Spatiotemporal expression of zebrafish keratin 18 during early embryogenesis and the establishment of a keratin 18:RFP transgenic line Gene Expr Patterns. 2006 Apr; 6(4):335-9. . View in PubMed

Foxd3 mediates zebrafish myf5 expression during early somitogenesis Dev Biol. 2006 Feb 15; 290(2):359-72. . View in PubMed

Expression, purification and DNA-binding activity of tilapia muscle-specific transcription factor, MyoD, produced in Escherichia coli Comp Biochem Physiol B Biochem Mol Biol. 2002 Apr; 131(4):795-805. . View in PubMed

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