In the United States there has been a rapid increase in the disease burden caused by antibiotic resistant members of the ESKAPE pathogens: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species. More than 2,000,000 illnesses and 23,000 deaths were attributed to antibiotic resistant infections in the US in 2013, with an added $20 billion dollar cost to the healthcare system. Continued research is needed to understand antibiotic tolerance mechanisms for the purpose of developing the next generation of therapeutics that can subvert these mechanisms.
I am interested in the study of antibiotic tolerance mechanisms by bacterial pathogens; including both antibiotic resistance and antibiotic persister cell formation. Better understanding of antibiotic tolerance mechanisms will guide improved therapeutic strategies for combating these infections. We use multiple research strategies including synthetic biology, microbial genetics, next-generation sequencing, and preclinical mouse models in our work.
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Monoclonal Antibody Protects Against Acinetobacter baumannii Infection by Enhancing Bacterial Clearance and Evading Sepsis. J Infect Dis. 2017 Aug 15; 216(4):489-501. View in: PubMed
Evaluation of serotypes 5 and 8 capsular polysaccharides in protection against Staphylococcus aureus in murine models of infection. Hum Vaccin Immunother. 2017 Jul 03; 13(7):1609-1614. View in: PubMed
Selectable Markers for Use in Genetic Manipulation of Extensively Drug-Resistant (XDR) Acinetobacter baumannii HUMC1. mSphere. 2017 Mar-Apr; 2(2). View in: PubMed
Clinical and Pathophysiological Overview of Acinetobacter Infections: a Century of Challenges. Clin Microbiol Rev. 2017 Jan; 30(1):409-447. View in: PubMed
Cathepsin K Contributes to Cavitation and Collagen Turnover in Pulmonary Tuberculosis. J Infect Dis. 2016 Feb 15; 213(4):618-27. View in: PubMed
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Mycobacterium tuberculosis dysregulates MMP/TIMP balance to drive rapid cavitation and unrestrained bacterial proliferation. J Pathol. 2015 Feb; 235(3):431-44. View in: PubMed
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Computer-aided detection and quantification of cavitary tuberculosis from CT scans. Med Phys. 2013 Nov; 40(11):113701. View in: PubMed
A computational pipeline for quantification of pulmonary infections in small animal models using serial PET-CT imaging. EJNMMI Res. 2013 Jul 23; 3(1):55. View in: PubMed
Gene expression of Mycobacterium tuberculosis putative transcription factors whiB1-7 in redox environments. PLoS One. 2012; 7(7):e37516. View in: PubMed
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