Faculty

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Daniel Philipp Holschneider, MD
Professor of Psychiatry & The Behavioral Sciences
Assistant Director of Consult/Liaison Psychiatry For LAC+USC
Psychiatry and The Behavioral Sciences
MCA B1 1333 San Pablo Street Health Sciences Campus Los Angeles
+1 323 442 1536

Overview

My laboratory's primary interest over the past 15 years has been the brain imaging of rodent behaviors as they occur in the nontethered, nonrestrained animal. A primary tool has been perfusion based mapping using the classic autoradiographic methods. Unique about our methods is that they allow us to evaluate changes in functional brain activation at the circuit level across the entire brain. Perfusion or metabolic mapping, as we have proposed, fills a gap in the current armamentarium of imaging tools in that it can deliver a 3D assessment of functional activation of the awake, nonrestrained animal, with a spatial resolution of 100 microns.
We have made significant contributions to the methodology in several areas (a) adapting statistical parametric mapping and connectivity analyses to three-dimensional autoradiographic data sets of the rat and mouse brain, (b) developing methods to allow the generation of cortical flat maps in rats and, (c) developing an implantable minipump that allows radiotracer bolus administration by remote activation in the freely moving animal. An ongoing focus is the application of functional connectivity analyses [correlation matrices of regional changes in CBF, graph theoretical analysis of network ?hubs? (Pajek software), effective connectivity analyses using structural equation modeling].
The lab has broad experience evaluating behavioral function in rat and mouse models of pain, brain injury, and fear, as well as exercise. Extensive experience is available for vascular surgical procedures in rats, mice and rabbits, as well as expertise in physiologic monitoring (locomotor activity, telemetric monitoring of EMG, EEG, ECG, cardiac output).

The expertise of the laboratory include functional brain mapping, animal behavior, physiologic monitoring (EEG, EMG, EKG) and basic histochemistry.

Publications

Recruitment of prefrontal-striatal circuit in response to skilled motor challenge. Neuroreport. 2017 Sep 08. View in: PubMed

Effects of water avoidance stress on peripheral and central responses during bladder filling in the rat: A multidisciplinary approach to the study of urologic chronic pelvic pain syndrome (MAPP) research network study. PLoS One. 2017; 12(9):e0182976. View in: PubMed

Engaging cognitive circuits to promote motor recovery in degenerative disorders. exercise as a learning modality. J Hum Kinet. 2016 Sep 01; 52:35-51. View in: PubMed

Evidence of functional brain reorganization on the basis of blood flow changes in the CAG140 knock-in mouse model of Huntington's disease. Neuroreport. 2016 Jun 15; 27(9):632-9. View in: PubMed

Early life stress elicits visceral hyperalgesia and functional reorganization of pain circuits in adult rats. Neurobiol Stress. 2016 Jun 1; 3:8-22. View in: PubMed

Prone positioning decreases cardiac output and increases systemic vascular resistance in neonates. J Perinatol. 2015 Jun; 35(6):424-7. View in: PubMed

Recruitment of the prefrontal cortex and cerebellum in Parkinsonian rats following skilled aerobic exercise. Neurobiol Dis. 2015 May; 77:71-87. View in: PubMed

Neonatal stress from limited bedding elicits visceral hyperalgesia in adult rats. Neuroreport. 2015 Jan 07; 26(1):13-6. View in: PubMed

The Effects of Exercise on Dopamine Neurotransmission in Parkinson's Disease: Targeting Neuroplasticity to Modulate Basal Ganglia Circuitry. Brain Plast. 2015; 1(1):29-39. View in: PubMed

Exercise alters resting-state functional connectivity of motor circuits in parkinsonian rats. Neurobiol Aging. 2015 Jan; 36(1):536-44. View in: PubMed

Exercise training reinstates cortico-cortical sensorimotor functional connectivity following striatal lesioning: development and application of a subregional-level analytic toolbox for perfusion autoradiographs of the rat brain. Front Phys. 2014 Dec 03; 2. View in: PubMed

Functional connectivity-based parcellation and connectome of cortical midline structures in the mouse: a perfusion autoradiography study. Front Neuroinform. 2014; 8:61. View in: PubMed

Remote brain network changes after unilateral cortical impact injury and their modulation by acetylcholinesterase inhibition. J Neurotrauma. 2013 Jun 01; 30(11):907-19. View in: PubMed

Functional reorganization of motor and limbic circuits after exercise training in a rat model of bilateral parkinsonism. PLoS One. 2013; 8(11):e80058. View in: PubMed

Alterations in prefrontal-limbic functional activation and connectivity in chronic stress-induced visceral hyperalgesia. PLoS One. 2013; 8(3):e59138. View in: PubMed

Acetylcholinesterase inhibition interacts with training to reverse spatial learning deficits after cortical impact injury. J Neurotrauma. 2012 Oct 10; 29(15):2457-64. View in: PubMed

Circadian rhythmicity in serotonin transporter knockout mice. Life Sci. 2012 Oct 05; 91(11-12):365-8. View in: PubMed

Ceftriaxone upregulates the glutamate transporter in medial prefrontal cortex and blocks reinstatement of methamphetamine seeking in a condition place preference paradigm. Brain Res. 2012 May 25; 1456:14-21. View in: PubMed

Anxiolytic-like effect of pregabalin on unconditioned fear in the rat: an autoradiographic brain perfusion mapping and functional connectivity study. Neuroimage. 2012 Feb 15; 59(4):4168-88. View in: PubMed

Functional brain activation during retrieval of visceral pain-conditioned passive avoidance in the rat. Pain. 2011 Dec; 152(12):2746-56. View in: PubMed

Acetylcholinesterase inhibition and locomotor function after motor-sensory cortex impact injury. J Neurotrauma. 2011 Sep; 28(9):1909-19. View in: PubMed

Antenatal maternal stress alters functional brain responses in adult offspring during conditioned fear. Brain Res. 2011 Apr 18; 1385:163-74. View in: PubMed

The role of exercise in facilitating basal ganglia function in Parkinson's disease. Neurodegener Dis Manag. 2011 Apr 01; 1(2):157-170. View in: PubMed

The role of experimental models in developing new treatments for irritable bowel syndrome. Expert Rev Gastroenterol Hepatol. 2011 Feb; 5(1):43-57. View in: PubMed

Mapping functional brain activation using [14C]-iodoantipyrine in male serotonin transporter knockout mice. PLoS One. 2011; 6(8):e23869. View in: PubMed

Powering an implantable minipump with a multi-layered printed circuit coil for drug infusion applications in rodents. Ann Biomed Eng. 2010 Mar; 38(3):707-13. View in: PubMed

A low power, on demand electrothermal valve for wireless drug delivery applications. Lab Chip. 2010 Jan 07; 10(1):101-10. View in: PubMed

Enhancing neuroplasticity in the basal ganglia: the role of exercise in Parkinson's disease. Mov Disord. 2010; 25 Suppl 1:S141-5. View in: PubMed

A Parylene MEMS Electrothermal Valve. J Microelectromech Syst. 2009 Dec; 18(6):1184-1197. View in: PubMed

Estimation of indocyanine green concentration in blood from fluorescence emission: application to hemodynamic assessment during hemodialysis. J Biomed Opt. 2009 Sep-Oct; 14(5):054006. View in: PubMed

Snap-frozen brain tissue sections stored with desiccant at ambient laboratory conditions without chemical fixation are resistant to degradation for a minimum of 6 months. Appl Immunohistochem Mol Morphol. 2009 Mar; 17(2):165-71. View in: PubMed

Studying the brain-gut axis with pharmacological imaging. Ann N Y Acad Sci. 2008 Nov; 1144:256-64. View in: PubMed

Memory, mood, dopamine, and serotonin in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-lesioned mouse model of basal ganglia injury. Neurobiol Dis. 2008 Nov; 32(2):319-27. View in: PubMed

Changes in regional brain perfusion during functional brain activation: comparison of [(64)Cu]-PTSM with [(14)C]-Iodoantipyrine. Brain Res. 2008 Oct 09; 1234:32-43. View in: PubMed

Regional brain activation in conscious, nonrestrained rats in response to noxious visceral stimulation. Pain. 2008 Aug 15; 138(1):233-43. View in: PubMed

Brain maps on the go: functional imaging during motor challenge in animals. Methods. 2008 Aug; 45(4):255-61. View in: PubMed

Flattened cortical maps of cerebral function in the rat: a region-of-interest approach to data sampling, analysis and display. Neurosci Lett. 2008 Mar 28; 434(2):179-84. View in: PubMed

Reorganization of functional brain maps after exercise training: Importance of cerebellar-thalamic-cortical pathway. Brain Res. 2007 Dec 12; 1184:96-107. View in: PubMed

Changes in brain functional activation during resting and locomotor states after unilateral nigrostriatal damage in rats. Neuroimage. 2007 Jul 1; 36(3):755-73. View in: PubMed

Fluorescence dilution technique for measurement of cardiac output and circulating blood volume in healthy human subjects. Anesthesiology. 2007 Mar; 106(3):491-8. View in: PubMed

Transcutaneous RF-powered implantable minipump driven by a class-E transmitter. IEEE Trans Biomed Eng. 2006 Aug; 53(8):1705-8. View in: PubMed

Mapping cerebral blood flow changes during auditory-cued conditioned fear in the nontethered, nonrestrained rat. Neuroimage. 2006 Feb 15; 29(4):1344-58. View in: PubMed

In vivo quantitative assessment of catheter patency in rats. Lab Anim. 2005 Jul; 39(3):259-68. View in: PubMed

Transcutaneous fluorescence dilution cardiac output and circulating blood volume during hemorrhagic hypovolemia. Anesthesiology. 2005 Apr; 102(4):774-82. View in: PubMed

A spontaneous point mutation produces monoamine oxidase A/B knock-out mice with greatly elevated monoamines and anxiety-like behavior. J Biol Chem. 2004 Sep 17; 279(38):39645-52. View in: PubMed

Statistical parametric mapping applied to an autoradiographic study of cerebral activation during treadmill walking in rats. Neuroimage. 2004 Sep; 23(1):252-9. View in: PubMed

Mapping brain function in freely moving subjects. Neurosci Biobehav Rev. 2004 Sep; 28(5):449-61. View in: PubMed

Measurement of cardiac output with indocyanine green transcutaneous fluorescence dilution technique. Anesthesiology. 2004 Jun; 100(6):1476-83. View in: PubMed

Striatal damage and oxidative stress induced by the mitochondrial toxin malonate are reduced in clorgyline-treated rats and MAO-A deficient mice. Neurochem Res. 2004 Apr; 29(4):741-6. View in: PubMed

Activation of cerebral cortex during acoustic challenge or acute foot-shock in freely moving, nontethered rats. Neurosci Lett. 2004 Jan 02; 354(1):74-8. View in: PubMed

Functional brain mapping in freely moving rats during treadmill walking. J Cereb Blood Flow Metab. 2003 Aug; 23(8):925-32. View in: PubMed

An implantable bolus infusion pump for use in freely moving, nontethered rats. Am J Physiol Heart Circ Physiol. 2002 Oct; 283(4):H1713-9. View in: PubMed

Heart rate dynamics in monoamine oxidase-A- and -B-deficient mice. Am J Physiol Heart Circ Physiol. 2002 May; 282(5):H1751-9. View in: PubMed

Increased baroreceptor response in mice deficient in monoamine oxidase A and B. Am J Physiol Heart Circ Physiol. 2002 Mar; 282(3):H964-72. View in: PubMed

Fluorescence of indocyanine green in blood: intensity dependence on concentration and stabilization with sodium polyaspartate. J Photochem Photobiol B. 2001 Dec 31; 65(2-3):157-64. View in: PubMed

Biochemical, behavioral, physiologic, and neurodevelopmental changes in mice deficient in monoamine oxidase A or B. Brain Res Bull. 2001 Nov 15; 56(5):453-62. View in: PubMed

Genotype to phenotype: challenges and opportunities. Int J Dev Neurosci. 2000 Oct; 18(6):615-8. View in: PubMed

Attenuation of brain high frequency electrocortical response after thiopental in early stages of Alzheimer's dementia. Psychopharmacology (Berl). 2000 Mar; 149(1):6-11. View in: PubMed

Regional cerebral cortical activation in monoamine oxidase A-deficient mice: differential effects of chronic versus acute elevations in serotonin and norepinephrine. Neuroscience. 2000; 101(4):869-77. View in: PubMed

Changes in electrocortical power and coherence in response to the selective cholinergic immunotoxin 192 IgG-saporin. Exp Brain Res. 1999 May; 126(2):270-80. View in: PubMed

Cerebral cortical blood flow maps are reorganized in MAOB-deficient mice. Brain Res. 1999 Apr 3; 824(1):36-44. View in: PubMed

Selective immunotoxin-induced cholinergic deafferentation alters blood flow distribution in the cerebral cortex. Brain Res. 1999 Feb 6; 818(1):1-11. View in: PubMed

Lack of protection from ischemic injury of monoamine oxidase B-deficient mice following middle cerebral artery occlusion. Neurosci Lett. 1999 Jan 15; 259(3):161-4. View in: PubMed

Clinical neurophysiology using electroencephalography in geriatric psychiatry: neurobiologic implications and clinical utility. J Geriatr Psychiatry Neurol. 1999; 12(3):150-64. View in: PubMed

Lack of protection of monoamine oxidase B-deficient mice from age-related spatial learning deficits in the Morris water maze. Life Sci. 1999; 65(17):1757-63. View in: PubMed

Effects of ovariectomy on cerebral flow of rats. Neuroendocrinology. 1998 Apr; 67(4):260-8. View in: PubMed

Effects of cholinergic deafferentation and NGF on brain electrical coherence. Brain Res Bull. 1998 Mar 15; 45(5):531-41. View in: PubMed

Tissue-specific effects of estrogen on monoamine oxidase A and B in the rat. Life Sci. 1998; 63(3):155-60. View in: PubMed

Changes in cortical EEG and cholinergic function in response to NGF in rats with nucleus basalis lesions. Brain Res. 1997 Aug 15; 765(2):228-37. View in: PubMed

Loss of high-frequency brain electrical response to thiopental administration in Alzheimer's-type dementia. Neuropsychopharmacology. 1997 Apr; 16(4):269-75. View in: PubMed

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