The Molecular Genomics Core (MGC) offers high-throughput analyses of genetic, epigenetic and transcriptomic variation. The MGC was created in the summer of 2009 through the merger of the DNA Core, the Microarray Core and the Genomics Core. The MGC is divided into an HSC (Health Science Campus) component and a CHLA (Children’s Hospital, Los Angeles) component.
Major Services, Technologies, Major Equipment and Expertise Provided
Consultation. Users are encouraged to meet with the core director or co-director, as appropriate, prior to project initiation to discuss their needs for the proposed project. The goal of this consultation is to educate the core about the researcher’s needs and to educate the user about possible approaches and services.
Direct Services. Direct services provided by the MGC include sample preparation and analytical assays. Sample preparation is performed on research samples using good laboratory practices or from clinical samples in a CAP/CLIA-certified laboratory for clinical research and diagnostics.
Sample preparation. Biospecimen processing is available for research (HSC) and clinical (CHLA) samples from body fluids, fresh and archival tissue, and non-mammalian sources (i.e. plasmid/phage DNA for capillary sequencing).
- DNA isolation. High-throughput DNA isolation from blood, buffy coat, buccal cells (swabs and mouthwash), saliva, serum, semen and paraffin-embedded tissue is available through the core. A majority of the DNA isolation chemistry utilizes magnetic bead-based isolation kits available to run on the Maxwell Particle Movers using < 400 ul of sample. All purified DNA is transferred into either 1D or 2D barcoded tubes for storage and is typically divided into at least two aliquots for storage in separate containers.
- NA isolation. The core encourages users to collect specimens in specially designed containers to stabilize source RNA at the time of collection for whole blood (PAXgene RNA Blood Vacutainers), from saliva collections (RNAprotect Saliva Reagent), or from solid tissue (RNAlater Tissue Protect Tubes). For formalin-fixed, paraffin-embedded (FFPE) samples, blocks are resectioned, a pilot section is stained, and a representative area is chosen by a consulting pathologist that is then cored with a 2 mm punch. The extracted core is immediately placed in RNA extraction medium for further processing