{"id":9279,"date":"2025-09-16T06:30:00","date_gmt":"2025-09-16T13:30:00","guid":{"rendered":"https:\/\/keck.usc.edu\/news\/?p=9279"},"modified":"2025-09-17T09:25:28","modified_gmt":"2025-09-17T16:25:28","slug":"stem-cell-transplant-for-stroke-leads-to-brain-cell-growth-and-functional-recovery-in-mice","status":"publish","type":"post","link":"https:\/\/keck.usc.edu\/news\/stem-cell-transplant-for-stroke-leads-to-brain-cell-growth-and-functional-recovery-in-mice\/","title":{"rendered":"Stem cell transplant for stroke leads to brain cell growth and functional recovery in mice"},"content":{"rendered":"\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--article-hero \"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--article-hero\"\n    \n      >\n\n    \n  <div class=\"text-container\">\n              \n<div class=\"f--field f--eyebrow\">\n\n    \n  <span>Press Release<\/span>\n\n\n\n<\/div>\n    \n              \n<div class=\"f--field f--page-title\">\n\n    \n      <h1>Stem cell transplant for stroke leads to brain cell growth and functional recovery in mice<\/h1>\n\n\n<\/div>\n    \n              \n<div class=\"f--field f--description\">\n\n    \n  <p>Insights about brain cell damage after stroke and repair after transplant could pave the way for therapies that extend the treatment window, as revealed in a lab study led by the Keck School of Medicine of USC.<\/p>\n\n\n\n<\/div>\n    \n          <div class=\"meta\">\n                  <span class=\"author\">Wayne Lewis<\/span>\n        \n                  <span class=\"date\">September 16, 2025<\/span>\n              <\/div>\n    \n              \n<div class=\"f--field f--embed\">\n\n    \n  <div class=\"heateor_sss_sharing_container heateor_sss_horizontal_sharing\" data-heateor-ss-offset=\"0\" data-heateor-sss-href='https:\/\/keck.usc.edu\/news\/stem-cell-transplant-for-stroke-leads-to-brain-cell-growth-and-functional-recovery-in-mice\/'><div class=\"heateor_sss_sharing_ul\"><a 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2.75z\"\/><\/svg><\/span><\/a><\/div><div class=\"heateorSssClear\"><\/div><\/div>\n\n\n<\/div>\n        \n  <\/div>\n\n          \n<div class=\"f--field f--image\">\n\n    \n    \n    \n        <figure>\n    \n    \n    \n              \n      <img\n                            data-src=\"https:\/\/keck.usc.edu\/news\/wp-content\/uploads\/sites\/68\/2025\/09\/iStock-1369757487-768x432.jpg\"\n          data-srcset=\"https:\/\/keck.usc.edu\/news\/wp-content\/uploads\/sites\/68\/2025\/09\/iStock-1369757487-1920x1080.jpg 1920w,https:\/\/keck.usc.edu\/news\/wp-content\/uploads\/sites\/68\/2025\/09\/iStock-1369757487-1280x720.jpg 1280w,https:\/\/keck.usc.edu\/news\/wp-content\/uploads\/sites\/68\/2025\/09\/iStock-1369757487-768x432.jpg 768w\"          data-sizes=\"(min-width:1200px) 75vw, (min-width:768px) 83vw, 100vw\"          class=\"lazyload\"\n        \n                alt=\"X- Ray illustration of brain stroke - 3d Illustration\"\n\n                \n        \n                \n                                      \/>\n\n    \n          <figcaption><p>Photo\/iStock<\/p>\n<\/figcaption>\n    <\/figure>\n    \n  \n  \n\n<\/div>\n  \n\n  <\/div><\/div>\n\n\n\n\n  \n    \n\n\n\n\n\n\n<div\n  class=\"cc--component-container cc--rich-text white\"\n\n  \n  \n  \n  \n  \n  \n  >\n  <div class=\"c--component c--rich-text\"\n    \n      >\n\n    \n  <div class=\"inner-wrapper\">\n        \n<div class=\"f--field f--wysiwyg\">\n\n    \n  <p><span style=\"font-weight: 400\">When someone has a stroke \u2014 a leading worldwide cause of death and disability \u2014 time is of the essence. Almost nine out of 10 cases are ischemic strokes, caused by restricted blood flow in the brain, and the current gold-standard treatment that breaks up blood clots must be delivered within four and a half hours of symptoms appearing.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400\"><\/p>\n<div style=\"float: right;width: 240px;height: 427px;margin-left: 15px;margin-bottom:10px\">\n<iframe loading=\"lazy\" src=\"https:\/\/www.youtube.com\/embed\/W0Ac5QvCQ3U\" width=\"100%\" height=\"100%\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><br \/>\n<\/iframe>\n<\/div>\n<p>Researchers are on the hunt for ways to extend that ticking clock and enable better stroke recovery. One promising prospect is an experimental stem cell therapy to help repair damaged brain tissue, co-developed by scientists at the <\/span><a href=\"https:\/\/keck.usc.edu\/\"><span style=\"font-weight: 400\">Keck School of Medicine of USC<\/span><\/a><span style=\"font-weight: 400\">, the University of Zurich and ETH Zurich in Switzerland. A study in the journal <\/span><a href=\"https:\/\/www.nature.com\/articles\/s41467-025-63725-3\"><span style=\"font-weight: 400\">Nature Communications<\/span><\/a><span style=\"font-weight: 400\"> showed that a stem cell transplant performed one week after an ischemic stroke in mice led to recovery.<\/span><\/p>\n<p><span style=\"font-weight: 400\">\u201cThere are a lot of patients who cannot get the acute treatment, and their blood vessels remain blocked,\u201d said co-corresponding author <a href=\"https:\/\/keck.usc.edu\/faculty-search\/ruslan-rust\/\">Ruslan Rust, PhD<\/a>, assistant professor of research physiology and neuroscience at the Keck School of Medicine of USC. \u201cIf we can bring this treatment to the clinic in the future, it may help patients who have long-term symptoms or large strokes see recovery.\u201d<\/span><\/p>\n<p><b>Employing stem cells to heal damaged brain tissue\u00a0<\/b><\/p>\n<p><span style=\"font-weight: 400\">Rust and his colleagues reprogrammed human blood cells into neural stem cells \u2014 which can mature into neurons \u2014 and transplanted them into the damaged brain tissue of mice that had strokes. After five weeks, the researchers compared their recovery to a group of mice from the same litter that had strokes but underwent surgery without transplantation.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400\">The brains of the mice that received transplanted neural stem cells showed more robust signs of recovery than those of untreated mice. The transplant recipients\u2019 brains had less inflammation, more growth of neurons and blood vessels, and more connectivity among neurons than the brains of the mice that did not receive transplanted cells. The treated mice also had less leakage from the blood-brain barrier, which is important for normal brain function and acts as a filter to keep harmful substance out of the brain.<\/span><\/p>\n<p><span style=\"font-weight: 400\">To measure function, the researchers used artificial intelligence to closely track the movement of the animals\u2019 limbs while walking and climbing up a ladder with irregular rungs.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400\">\u201cRecovery can be hard to determine in mice, so we needed to see these little differences,\u201d Rust said. \u201cThe unbiased view we got through this deep learning tool gave us a lot more detail about this complex process.\u201d<\/span><\/p>\n<p><span style=\"font-weight: 400\">The team found that treated mice fully recovered the fine motor skills tested in the climbing task five weeks after the transplants. By the end of the study, their gait also improved significantly compared to mice that received sham surgery.<\/span><\/p>\n<p><b>Clues among the new brain cells that develop<\/b><\/p>\n<p><span style=\"font-weight: 400\">When the researchers looked at which types of cells died off due to stroke, they found roughly a 50% reduction in neurons that secrete <\/span><span style=\"font-weight: 400\">gamma-aminobutyric acid (GABA),<\/span> <span style=\"font-weight: 400\">which decreases activity in the brain cells to which it binds. These GABA-secreting neurons, known as <\/span><span style=\"font-weight: 400\">GABAergic neurons, have<\/span> <span style=\"font-weight: 400\">previously been shown to assist stroke recovery.<\/span><\/p>\n<p><span style=\"font-weight: 400\">The team also explored the fate of the transplanted stem cells, finding that the majority had become GABAergic neurons. This is a possible indication that the local environment where the stroke injured the brain may help steer the development of the neural stem cells.<\/span><\/p>\n<p><span style=\"font-weight: 400\">Rust and his colleagues also analyzed the interactions between the transplanted cells and other cells in the brains of the mice. They found strong activity in several signaling pathways that were shown in prior studies to be associated with regenerating neurons, forming connections between neurons, and guiding how neurons branch out.<\/span><\/p>\n<p><span style=\"font-weight: 400\">\u201cMechanistic insight can be quite important if we seek to inform new therapies or improve emerging ones,\u201d Rust said. \u201cUnderstanding the mechanisms allows us to think about adapting a drug that regulates them \u2014 perhaps one that\u2019s already clinically approved for a different disease. It could open up a whole new wave of therapies.\u201d<\/span><\/p>\n<p><span style=\"font-weight: 400\">The team is currently investigating other ways to increase activity in the pathways identified in the study and evaluating the results of the transplant in mice for periods longer than five weeks.<\/span><\/p>\n<p><span style=\"font-weight: 400\">\u201cIf we can help people by transplanting stem cells into a human stroke patient, we want the cells to be there for the rest of their life,\u201d Rust said. \u201cSo our aim would be to look across the whole lifetime of a mouse and see what happens with the cells, and also see whether this recovery is sustained or even improves.\u201d\u00a0<\/span><\/p>\n<p><b>About this study\u00a0<\/b><\/p>\n<p><span style=\"font-weight: 400\">The study\u2019s first author is Rebecca Weber of the University of Zurich and ETH Zurich. The co-corresponding author is Christian Tackenberg of the University of Zurich and ETH Zurich. Other co-authors are Beatriz Ach\u00f3n Buil, Nora Rentsch, Patrick Perron, Stefanie Halliday, Chantal Bodenmann, Kathrin Z\u00fcrcher, Daniela Uhr, Debora Meier, Siri Peter, Melanie Generali of the University of Zurich and ETH Zurich; Allison Bosworth, Mingzi Zhang and Kassandra Kisler of the Keck School of Medicine; Shuo Lin and Markus R\u00fcegg of the University of Basel in Switzerland; and Roger Nitsch of Neurimmune, a Swiss biopharmaceutical company.<\/span><\/p>\n<p><span style=\"font-weight: 400\">The study received support from the Swiss 3R Competence Center; the Swiss National Science Foundation; the Neuroscience Center Zurich at the University of Zurich and ETH Zurich; and the Maxi Foundation.<\/span><\/p>\n\n\n\n<\/div>\n  <\/div>\n\n\n  <\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":278,"featured_media":9280,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":true,"advgb_blocks_editor_width":"","advgb_blocks_columns_visual_guide":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[476,446,169,436,632],"class_list":["post-9279","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-press-release","tag-brain-health","tag-homepage","tag-latest","tag-usc-stem-cell","tag-usc-zilkha-neurogenetic-institute"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v28.2 (Yoast SEO v28.6) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Stem cell transplant for stroke leads to brain cell growth and functional recovery in mice<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, 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