Faculty

Robert A Farley, PhD
Robert A Farley, PhD
Professor of Physiology & Biophysics
Physiology and Biophysics
MCH 250, 1333 San Pablo Street Health Sciences Campus Los Angeles
Ph.D. (1975; Biophysics) from the University of Rochester, Postdoctoral fellowships at Yale University (1975-1976; Molecular Biophysics and Biochemistry) and Harvard University (1976-1980; Biochemistry and Molecular Biology). Joined USC faculty in 1980; currently Professor of Physiology and Biophysics, and of Biochemistry and Molecular Biology. Visiting faculty sabbatical appointments at the University of Virginia (Chemistry; 1992), Caltech (Biology; 1998-2000), and the University of Calgary (Centre for Molecular Simulations; 2015).

K+ Block Is the Mechanism of Functional Asymmetry in Bacterial Na(v) Channels PLoS Comput Biol. 2016 Jan; 12(1):e1004482. . View in PubMed

Non-equilibrium dynamics contribute to ion selectivity in the KcsA channel PLoS One. 2014; 9(1):e86079. . View in PubMed

Perspectives on: information coding in mammalian sensory physiology J Gen Physiol. 2011 Sep; 138(3):281-2. . View in PubMed

Calcium-activated endoplasmic reticulum stress as a major component of tumor cell death induced by 2,5-dimethyl-celecoxib, a non-coxib analogue of celecoxib Mol Cancer Ther. 2007 Apr; 6(4):1262-75. . View in PubMed

Evidence calcium pump binds magnesium before inorganic phosphate J Biol Chem. 2005 Mar 04; 280(9):7435-43. . View in PubMed

The role of loop 6/7 in folding and functional performance of Na,K-ATPase J Biol Chem. 2004 Oct 29; 279(44):45594-602. . View in PubMed

Specialized protective role of mucosal glutathione in pigmented rabbit conjunctiva Invest Ophthalmol Vis Sci. 2003 Oct; 44(10):4427-38. . View in PubMed

Mechanism of phosphoryl group transfer Ann N Y Acad Sci. 2003 Apr; 986:275-7. . View in PubMed

Site-directed mutagenesis of amino acids in the cytoplasmic loop 6/7 of Na,K-ATPase Ann N Y Acad Sci. 2003 Apr; 986:96-100. . View in PubMed

Voltage-dependent transient currents of human and rat 5-HT transporters (SERT) are blocked by HEPES and ion channel ligands FEBS Lett. 2002 Feb 27; 513(2-3):247-52. . View in PubMed

Sodium pump isoform expression in heart failure: implication for treatment Basic Res Cardiol. 2002; 97 Suppl 1:I25-30. . View in PubMed

The cardiac sodium pump: structure and function Basic Res Cardiol. 2002; 97 Suppl 1:I19-24. . View in PubMed

Ouabain and substrate affinities of human Na(+)-K(+)-ATPase alpha(1)beta(1), alpha(2)beta(1), and alpha(3)beta(1) when expressed separately in yeast cells Am J Physiol Cell Physiol. 2001 Oct; 281(4):C1355-64. . View in PubMed

All human Na(+)-K(+)-ATPase alpha-subunit isoforms have a similar affinity for cardiac glycosides Am J Physiol Cell Physiol. 2001 Oct; 281(4):C1336-43. . View in PubMed

18O-exchange evidence that mutations of arginine in a signature sequence for P-type pumps affect inorganic phosphate binding Biochemistry. 2001 May 29; 40(21):6361-70. . View in PubMed

A hybrid between Na+,K+-ATPase and H+,K+-ATPase is sensitive to palytoxin, ouabain, and SCH 28080 J Biol Chem. 2001 Jan 26; 276(4):2608-15. . View in PubMed

An intermediate state of the gamma-aminobutyric acid transporter GAT1 revealed by simultaneous voltage clamp and fluorescence J Gen Physiol. 2000 Apr; 115(4):491-508. . View in PubMed

Effects of local anaesthetics on the activity of the Na,K-ATPase of canine renal medulla Pharmacol Res. 2000 Jan; 41(1):1-7. . View in PubMed

Effects of general anaesthetics on the activity of the Na,K-ATPase of canine renal medulla Pharmacol Res. 1999 Dec; 40(6):469-73. . View in PubMed

Catalytic activity of an isolated domain of Na,K-ATPase expressed in Escherichia coli Biophys J. 1999 Jul; 77(1):258-66. . View in PubMed

Valine 904, tyrosine 898, and cysteine 908 in Na,K-ATPase alpha subunits are important for assembly with beta subunits J Biol Chem. 1998 Nov 06; 273(45):29400-5. . View in PubMed

Erythrocyte aggregation tendency and cellular properties in horse, human, and rat: a comparative study Am J Physiol. 1997 Dec; 273(6 Pt 2):H2604-12. . View in PubMed

Amino acids Val115-Ile126 of rat gastric H(+)-K(+)-ATPase confer high affinity for Sch-28080 to Na(+)-K(+)-ATPase Am J Physiol. 1997 May; 272(5 Pt 1):C1717-25. . View in PubMed

Na+-K+-ATPase alpha-subunit containing Q905-V930 of gastric H+-K+-ATPase alpha preferentially assembles with H+-K+-ATPase beta Am J Physiol. 1997 Mar; 272(3 Pt 1):C923-30. . View in PubMed

Site-directed mutagenesis of the sodium pump: analysis of mutations to amino acids in the proposed nucleotide binding site by stable oxygen isotope exchange Biochemistry. 1997 Jan 28; 36(4):941-51. . View in PubMed

Erythrocyte aggregation tendency and cellular properties in horse, human, and rat: a comparative study Am J Physiol. 1997 12; 273(6):H2604-12. . View in PubMed

Photoaffinity labeling of the active site of the Na+/K(+)-ATPase with 4-azido-2-nitrophenyl phosphate Biochemistry. 1996 Jan 09; 35(1):47-55. . View in PubMed

The influence of beta subunit structure on the interaction of Na+/K(+)-ATPase complexes with Na+A chimeric beta subunit reduces the Na+ dependence of phosphoenzyme formation from ATP. J Biol Chem. 1995 Jun 09; 270(23):13937-47. . View in PubMed

Significance of sodium pump isoforms in digitalis therapy J Mol Cell Cardiol. 1995 Apr; 27(4):1001-9. . View in PubMed

Subunit requirements for expression of functional sodium pumps in yeast cells Biochim Biophys Acta. 1994 Aug 03; 1193(2):226-34. . View in PubMed

Identification of an amino acid in the ATP binding site of Na+/K(+)-ATPase after photochemical labeling with 8-azido-ATP Biochemistry. 1994 Apr 12; 33(14):4140-7. . View in PubMed

Photochemical labeling and inhibition of Na,K-ATPase by 2-Azido-ATPIdentification of an amino acid located within the ATP binding site. J Biol Chem. 1994 Mar 04; 269(9):6558-65. . View in PubMed

The influence of beta subunit structure on the stability of Na+/K(+)-ATPase complexes and interaction with K+ J Biol Chem. 1994 Mar 04; 269(9):6550-7. . View in PubMed

Simultaneous binding of phosphate and TNP-ADP to FITC-modified NA+,K(+)-ATPase Biochemistry. 1993 Sep 21; 32(37):9592-9. . View in PubMed

Regulation of Na,K-ATPase activity Curr Opin Nephrol Hypertens. 1993 Sep; 2(5):725-34. . View in PubMed

Influence of calcium permeabilization and membrane-attached hemoglobin on erythrocyte deformability Am J Hematol. 1992 Nov; 41(3):170-7. . View in PubMed

High-affinity ouabain binding by yeast cells expressing Na+, K(+)-ATPase alpha subunits and the gastric H+, K(+)-ATPase beta subunit Proc Natl Acad Sci U S A. 1992 Apr 01; 89(7):2834-8. . View in PubMed

Lysine 480 is not an essential residue for ATP binding or hydrolysis by Na,K-ATPase J Biol Chem. 1992 Feb 25; 267(6):3577-80. . View in PubMed

Temperature dependence of the rates of conformational changes reported by fluorescein 5'-isothiocyanate modification of H+,K(+)- and Na+,K(+)-ATPases Biochemistry. 1991 Apr 09; 30(14):3503-10. . View in PubMed

Regulation of testicular P-450 cholesterol side-chain cleavage and P-450 C17-20 lyase/C17 hydroxylase enzymes in the neonatal and adult rat Acta Endocrinol (Copenh). 1991 Apr; 124(4):449-54. . View in PubMed

The sodium pump needs its beta subunit FASEB J. 1990 Apr 01; 4(6):1598-605. . View in PubMed

Synthesis and assembly of functional mammalian Na,K-ATPase in yeast J Biol Chem. 1990 Mar 15; 265(8):4189-92. . View in PubMed

Beta subunit of (Na+ + K+)-ATPase contains three disulfide bonds Biochemistry. 1990 Feb 13; 29(6):1524-32. . View in PubMed

Ouabain-resistant (Na+,K+)-ATPase enzyme activity in chemically induced ouabain-resistant C3H/10T1/2 cells Mol Toxicol. 1989 Apr-Jun; 2(2):75-98. . View in PubMed

AAQUANT: a computer program for quantitative amino acid analysis of proteins and peptides Comput Biol Med. 1989; 19(6):453-9. . View in PubMed

All three potential N-glycosylation sites of the dog kidney (Na+ + K+)-ATPase beta-subunit contain oligosaccharide Biochim Biophys Acta. 1988 Apr 28; 954(1):50-7. . View in PubMed

Development of a heterologous gene expression system for the Na,K-ATPase subunits in the yeast Saccharomyces cerevisiae Prog Clin Biol Res. 1988; 268B:85-90. . View in PubMed

Molecular cloning and sequence analysis of the (Na+ + K+)-ATPase beta subunit from dog kidney Biochim Biophys Acta. 1987 Apr 08; 912(2):244-53. . View in PubMed

Inhibition of ion pump ATPase activity by 3'-O-(4-benzoyl)benzoyl-ATP (BzATP): assessment of BzATP as an active site-directed probe Biochim Biophys Acta. 1986 Aug 07; 860(1):9-14. . View in PubMed

The amino acid sequence of an active site peptide from the H,K-ATPase of gastric mucosa J Biol Chem. 1985 Apr 10; 260(7):3899-901. . View in PubMed

The amino acid sequence of a fluorescein-labeled peptide from the active site of (Na,K)-ATPase J Biol Chem. 1984 Aug 10; 259(15):9532-5. . View in PubMed

The active site structure of Na+- and K+-stimulated ATPaseLocation of a specific fluorescein isothiocyanate reactive site. J Biol Chem. 1982 May 25; 257(10):5601-6. . View in PubMed

Location of binding sites on the (Na,K)-ATPase for fluorescein-5'-isothiocyanate and ouabain Ann N Y Acad Sci. 1982; 402:289-91. . View in PubMed

Identification of regions of the catalytic subunit of (Na-K)-ATPase embedded within the cell membranePhotochemical labeling with [3H]adamantane diazirine. J Biol Chem. 1980 Feb 10; 255(3):860-4. . View in PubMed

Proteolytic fragmentation of the catalytic subunit of the sodium and potassium adenosine triphosphataseAlignment of tryptic and chymotryptic fragments and location of sites labeled with ATP and iodoacetate. J Biol Chem. 1979 Apr 10; 254(7):2221-8. . View in PubMed

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