Julio A. Camarero Palao, PhD


Image of Julio A. Camarero Palao, PhD
Is this your profile? Click to edit


Dr. Camarero started his studies in chemistry at the University if Barcelona (Spain), received his Master degree in 1992, and finished his PhD thesis there in 1996. Afterwards he joined the group of Professor Tom W. Muir at The Rockefeller University as a Burroughs Wellcome Fellow where he contributed to the development of new chemoselective ligation techniques for the chemical engineering of proteins. In 2000, he moved to the Lawrence Livermore National Laboratory as a Distinguished Lawrence Fellow where he became staff scientist and head of laboratory in 2003. He finally joined the University of Southern California in 2007 as an associate professor.

His current research interests are focused in the development of new bioorganic approaches using protein splicing and synthetic protein chemistry for studying biological processes involved in bacterial pathogenicity and how can be modulated or inhibited by small molecules. Dr. Camarero has authored over 40 peer-reviewed publications and four invited book chapters.


  • Resistance is futile: targeting multidrug-resistant bacteria with de novo Cys-rich cyclic polypeptides RSC Chem Biol. 2023 Oct 04; 4(10):722-735. . View in PubMed
  • The Potential of the Cyclotide Scaffold for Drug Development Biomedicines. 2019 Apr 19; 7(2). . View in PubMed
  • J Med Chem. 2017 03 09; 60(5):1916-1927. . View in PubMed
  • Cyclotides: Overview and biotechnological applications Chembiochem. 2017; 18(14):XXX-XXX. . View in PubMed
  • Protein Chemical Modification Inside Living Cells Using Split Inteins Methods Mol Biol. 2017; 1495:111-130. . View in PubMed
  • Recombinant Expression of Cyclotides Using Split Inteins Methods Mol Biol. 2017; 1495:41-55. . View in PubMed
  • Cyclotides, a versatile ultrastable micro-protein scaffold for biotechnological applications Bioorg Med Chem Lett. 2017 12 01; 27(23):5089-5099. . View in PubMed
  • Efficient recombinant expression of SFTI-1 in bacterial cells using intein-mediated protein trans-splicing Biopolymers. 2016 Nov; 106(6):818-824. . View in PubMed
  • Design of a MCoTI-Based Cyclotide with Angiotensin (1-7)-Like Activity Molecules. 2016 Jan 26; 21(2):152. . View in PubMed
  • Recombinant Expression and Phenotypic Screening of a Bioactive Cyclotide Against a-Synuclein-Induced Cytotoxicity in Baker’s Yeast Angew Chem Int Ed Engl. 2015 Jul 13; 54(29):8390-4. . View in PubMed
  • Rapid parallel synthesis of bioactive folded cyclotides by using a tea-bag approach Chembiochem. 2015 Mar 23; 16(5):827-33. . View in PubMed
  • Chemical and biological production of cyclotides Adv Bot Res. 2015; 76:271-303. . View in PubMed
  • Changing the topology of protein backbone: the effect of backbone cyclization on the structure and dynamics of a SH3 domain Front Chem. 2015; 3:26. . View in PubMed
  • J Phys Chem B. 2014 Dec 11; 118(49):14257-66. . View in PubMed
  • Intein applications: from protein purification and labeling to metabolic control methods J Biol Chem. 2014 May 23; 289(21):14512-9. . View in PubMed
  • Recombinant expression of backbone-cyclized polypeptides Biopolymers. 2013 Sep; 100(5):502-9. . View in PubMed
  • In vivo activation of the p53 tumor suppressor pathway by an engineered cyclotide J Am Chem Soc. 2013 Aug 07; 135(31):11623-11633. . View in PubMed
  • Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions Angew Chem Int Ed Engl. 2013 Mar 11; 52(11):3126-31. . View in PubMed
  • Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature Nat Prod Rep. 2013 Jan; 30(1):108-60. . View in PubMed
  • Design of a novel cyclotide-based CXCR4 antagonist with anti-human immunodeficiency virus (HIV)-1 activity J Med Chem. 2012 Dec 13; 55(23):10729-34. . View in PubMed
  • Biological synthesis of circular polypeptides J Biol Chem. 2012 Aug 03; 287(32):27026-32. . View in PubMed
  • Bioorg Med Chem Lett. 2012 Apr 15; 22(8):2823-6. . View in PubMed
  • In-cell fluorescence activation and labeling of proteins mediated by FRET-quenched split inteins J Am Chem Soc. 2012 Apr 11; 134(14):6344-53. . View in PubMed
  • Mol Biosyst. 2012 Apr; 8(4):1359-65. . View in PubMed
  • Biosynthesis and antimicrobial evaluation of backbone-cyclized a-defensins Biochemistry. 2011 Dec 06; 50(48):10508-19. . View in PubMed
  • Novel peptide-based scaffolds for drug discovery Curr Pharm Des. 2011 Dec; 17(38):4224-5. . View in PubMed
  • Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery Curr Pharm Des. 2011 Dec; 17(38):4294-307. . View in PubMed
  • Cellular uptake of cyclotide MCoTI-I follows multiple endocytic pathways J Control Release. 2011 Oct 30; 155(2):134-43. . View in PubMed
  • Protein microarrays: novel developments and applications Pharm Res. 2011 Jul; 28(7):1480-99. . View in PubMed
  • Legume cyclotides shed light on the genetic origin of knotted circular proteins Proc Natl Acad Sci U S A. 2011 Jun 21; 108(25):10025-6. . View in PubMed
  • Biological activities of natural and engineered cyclotides, a novel molecular scaffold for peptide-based therapeutics Curr Mol Pharmacol. 2010 Nov; 3(3):153-63. . View in PubMed
  • Backbone dynamics of cyclotide MCoTI-I free and complexed with trypsin Angew Chem Int Ed Engl. 2010 Sep 17; 49(39):7030-4. . View in PubMed
  • Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein Chembiochem. 2010 Jul 05; 11(10):1368-72. . View in PubMed
  • In vivo biosynthesis of an Ala-scan library based on the cyclic peptide SFTI-1 Amino Acids. 2010 May; 38(5):1313-22. . View in PubMed
  • Cyclotides, a promising molecular scaffold for peptide-based therapeutics Biopolymers. 2010; 94(5):611-6. . View in PubMed
  • Expressed protein ligation: a resourceful tool to study protein structure and function Cell Mol Life Sci. 2009 Dec; 66(24):3909-22. . View in PubMed
  • Biosynthesis and biological screening of a genetically encoded library based on the cyclotide MCoTI-I Chembiochem. 2009 Nov 02; 10(16):2663-70. . View in PubMed
  • Optimizing the future for biotechnology therapies, the key role of protein engineering Adv Drug Deliv Rev. 2009 Sep 30; 61(11):897-8. . View in PubMed
  • “Splicing up” drug discoveryCell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides. Adv Drug Deliv Rev. 2009 Sep 30; 61(11):908-17. . View in PubMed
  • Motion of a DNA sliding clamp observed by single molecule fluorescence spectroscopy J Biol Chem. 2008 Aug 22; 283(34):22895-906. . View in PubMed
  • Recent developments in the site-specific immobilization of proteins onto solid supports Biopolymers. 2008; 90(3):450-8. . View in PubMed
  • Development of a cell-based fluorescence resonance energy transfer reporter for Bacillus anthracis lethal factor protease Anal Biochem. 2007 Oct 01; 369(1):60-70. . View in PubMed
  • Biosynthesis of a fully functional cyclotide inside living bacterial cells Chembiochem. 2007 Aug 13; 8(12):1363-6. . View in PubMed
  • Correlation spectroscopy of minor fluorescent species: signal purification and distribution analysis Biophys J. 2007 Mar 15; 92(6):2184-98. . View in PubMed
  • Applications of functional protein microarrays: identifying protein-protein interactions in an array format Methods Mol Biol. 2007; 385:121-30. . View in PubMed
  • Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing Angew Chem Int Ed Engl. 2006 Mar 03; 45(11):1726-9. . View in PubMed
  • Biosynthesis of the cyclotide Kalata B1 by using protein splicing Angew Chem Int Ed Engl. 2006 Jan 30; 45(6):973-6. . View in PubMed
  • Synthesis of proteins by native chemical ligation using Fmoc-based chemistry Protein Pept Lett. 2005 Nov; 12(8):723-8. . View in PubMed
  • Expressed protein ligation: a new tool for the biosynthesis of cyclic polypeptides Protein Pept Lett. 2005 Nov; 12(8):789-94. . View in PubMed
  • Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for “protein chip” fabrication J Am Chem Soc. 2004 Nov 17; 126(45):14730-1. . View in PubMed
  • Preparation of peptide p-nitroanilides using an aryl hydrazine resin Org Lett. 2004 Oct 14; 6(21):3801-4. . View in PubMed
  • Fmoc-based synthesis of peptide alpha-thioesters using an aryl hydrazine support J Org Chem. 2004 Jun 11; 69(12):4145-51. . View in PubMed
  • Fabrication of assembled virus nanostructures on templates of chemoselective linkers formed by scanning probe nanolithography J Am Chem Soc. 2003 Jun 11; 125(23):6848-9. . View in PubMed
  • Autoregulation of a bacterial sigma factor explored by using segmental isotopic labeling and NMR Proc Natl Acad Sci U S A. 2002 Jun 25; 99(13):8536-41. . View in PubMed
  • Peptide chemical ligation inside living cells: in vivo generation of a circular protein domain Bioorg Med Chem. 2001 Sep; 9(9):2479-84. . View in PubMed
  • Rescuing a destabilized protein fold through backbone cyclization J Mol Biol. 2001 May 18; 308(5):1045-62. . View in PubMed
  • Native chemical ligation of polypeptidesCurr Protoc Protein Sci. 2001 May; Chapter 18:Unit18. 4. . View in PubMed
  • Mapping the molecular interface between the sigma(70) subunit of Ecoli RNA polymerase and T4 AsiA. J Mol Biol. 2001 Mar 02; 306(4):631-42. . View in PubMed
  • Native-like cyclic peptide models of a viral antigenic site: finding a balance between rigidity and flexibility J Mol Recognit. 2000 Jan-Feb; 13(1):5-13. . View in PubMed
  • Introduction of unnatural amino acids into proteins using expressed protein ligation Biopolymers. 1999; 51(5):343-54. . View in PubMed
  • Studying receptor-ligand interactions using encoded amino acid scanning Biochemistry. 1998 May 19; 37(20):7487-95. . View in PubMed
  • Chemical ligation of unprotected peptides directly from a solid support J Pept Res. 1998 Apr; 51(4):303-16. . View in PubMed
  • A cyclic disulfide peptide reproduces in solution the main structural features of a native antigenic site of foot-and-mouth disease virus Int J Biol Macromol. 1997 Jun; 20(3):209-19. . View in PubMed
  • A large-scale evaluation of peptide vaccines against foot-and-mouth disease: lack of solid protection in cattle and isolation of escape mutants J Virol. 1997 Apr; 71(4):2606-14. . View in PubMed
  • Solution versus solid-phase cyclization strategies for large sidechain lactam-bridged peptides: a comparative study J Pept Sci. 1995 Jul-Aug; 1(4):241-50. . View in PubMed
  • Direct evaluation of the immunodominance of a major antigenic site of foot-and-mouth disease virus in a natural host Virology. 1995 Jan 10; 206(1):298-306. . View in PubMed
  • A highly divergent antigenic site of foot-and-mouth disease virus retains its immunodominance Viral Immunol. 1995; 8(1):11-8. . View in PubMed
  • Cyclic peptides as conformationally restricted models of viral antigens: application to foot-and-mouth disease virus Biomed Pept Proteins Nucleic Acids. 1995; 1(3):133-40. . View in PubMed
  • Cyclic disulfide model of the major antigenic site of serotype-C foot-and-mouth disease virusSynthetic, conformational and immunochemical studies. FEBS Lett. 1993 Aug 09; 328(1-2):159-64. . View in PubMed
  • Non-additive effects of multiple amino acid substitutions on antigen-antibody recognition Eur J Immunol. 1992 Jun; 22(6):1385-9. . View in PubMed